Goat IgG, Fab Native Protein

Images

 
SDS-Page: Goat IgG, Fab Native Protein [NBP1-97022] - SDS-PAGE of Goat IgG F(ab) Fragment (0001). Lane M: 5 uL Opal Prestained Marker. Lane 1: Reduced Goat IgG Whole Molecule. Lane 2: Reduced Goat IgG F(c) Fragment. ...read more

Product Details

Summary
Reactivity GtSpecies Glossary
Applications WB, ELISA
Concentration
1.0 mg/ml

Order Details

View Available Conjugates

Goat IgG, Fab Native Protein Summary

Description
Native Goat IgG, Fab protein.

Source: Goat Serum

Preparation
Method
This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Goat IgG and anti-Goat IgG F(ab')2. No reaction was observed against anti-Goat IgG F(c) or anti- Papain.
Protein/Peptide Type
Native Protein
Purity
Immunoelectrophoresis (IEP)

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • ELISA 1:100 - 1:2000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunoelectrophoresis (IEP)

Background

Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. This product possesses the F(ab) region possessing the epitope-recognition site, both heavy and light chains of the antibody molecule are present.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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