GNAL Antibody

Images

 
Western Blot: GNAL Antibody [NBP1-00840] - Analysis of GNAL Antibody at 1:500 dilutionLane1:MCF-7 whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:PC12 whole cell lysate
Immunohistochemistry-Paraffin: GNAL Antibody [NBP1-00840] - Paraffin-embedded human colorectal carcinoma tissue at 1:50.
Western Blot: GNAL Antibody [NBP1-00840] - Extracts from LOVO cells.
Immunohistochemistry-Paraffin: GNAL Antibody [NBP1-00840] - Immunohistochemistry (IHC) analyzes of Ga olf (D81) antibody in paraffin-embedded human brain tissue

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-00840
    • Availability
      Product Discontinued

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GNAL Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 50-100 of Human Galpha olf.
Clonality
Polyclonal
Host
Rabbit
Gene
GNAL
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of Galpha olf (D81) pAb in extracts from LOVO cells. Immunohistochemistry (IHC) analyzes of Galpha olf (D81) pAb in paraffin-embedded human brain tissue. Immunocytochemistry/Immunofluorescence was reported in scientific literature.
Theoretical MW
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP1-00840 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GNAL Antibody

  • Adenylate cyclase-stimulating G alpha protein, olfactory type
  • guanine nucleotide binding protein (G protein), alpha activating activitypolypeptide, olfactory typ
  • guanine nucleotide binding protein (G protein), alpha activating activitypolypeptide, olfactory type
  • guanine nucleotide binding protein (G protein), alpha stimulating activitypolypeptide, olfactory type
  • guanine nucleotide-binding protein G(olf) subunit alpha

Background

Heterotrimeric G proteins function to relay information from cell surface receptors to intracellular effectors. Each of a very broad range of receptors specifically detects an extracellular stimulus (a photon, pheromone, odorant, hormone or neurotransmitter) while the effectors (e.g. adenyl cyclase), which act to generate one or more intracellular messengers, are less numerous. In mammals, G protein alpha, beta and gamma polypeptides are encoded by at least 16, 4 and 7 genes, respectively. Most interest in G proteins has been focused on their alpha subunits, since these proteins bind and hydrolyze GTP, and most obviously regulate the activity of the best studied effectors. The Gs subfamily of Galpha subunits includes two closely related proteins, Galpha s and Galpha olf, which, respectively, stimulate adenylate cyclase and mediate response to olfactory stimuli.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GNAL Antibody (NBP1-00840)(1)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: ICC/IF.


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ICC/IF
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GNAL Antibody (NBP1-00840). (Showing 1 - 1 of 1 FAQ).

  1. I have a question about the GNAL antibody in question is NBP1-00840. Can you tell me what is known about this antibody's cross- reactivity with G alpha S? I am looking for an antibody that will detect G alpha olf specifically, and I note that the picture shows positive western blot in a human colon cancer cell line. Does the cell line express G alpha olf?
    • The cross-reactivity of NBP1-00840 and GNAS has not been evaluated. However, there is no homology between the immunogen for NBP1-00840 and the GNAS protein, therefore I do not expect there to be any interaction. In addition to our testing data, there is information in the literature about the expression of G alpha olf in colorectal cells as well as in protein databases. Please see Genecards and PubMed for additional information.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GNAL
Entrez