Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Phosphate buffered saline, pH 7.2.
0.05% Sodium Azide
Immunogen affinity purified
Western blot (WB) analysis of Galpha olf (D81) pAb in extracts from LOVO cells. Immunohistochemistry (IHC) analyzes of Galpha olf (D81) pAb in paraffin-embedded human brain tissue. Immunocytochemistry/Immunofluorescence was reported in scientific literature. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publication using NBP1-00840 in the following applications:
Adenylate cyclase-stimulating G alpha protein, olfactory type
guanine nucleotide binding protein (G protein), alpha activating activitypolypeptide, olfactory typ
guanine nucleotide binding protein (G protein), alpha activating activitypolypeptide, olfactory type
guanine nucleotide binding protein (G protein), alpha stimulating activitypolypeptide, olfactory type
guanine nucleotide-binding protein G(olf) subunit alpha
Heterotrimeric G proteins function to relay information from cell surface receptors to intracellular effectors. Each of a very broad range of receptors specifically detects an extracellular stimulus (a photon, pheromone, odorant, hormone or neurotransmitter) while the effectors (e.g. adenyl cyclase), which act to generate one or more intracellular messengers, are less numerous. In mammals, G protein alpha, beta and gamma polypeptides are encoded by at least 16, 4 and 7 genes, respectively. Most interest in G proteins has been focused on their alpha subunits, since these proteins bind and hydrolyze GTP, and most obviously regulate the activity of the best studied effectors. The Gs subfamily of Galpha subunits includes two closely related proteins, Galpha s and Galpha olf, which, respectively, stimulate adenylate cyclase and mediate response to olfactory stimuli.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for GNAL Antibody (NBP1-00840). (Showing 1 - 1 of 1 FAQ).
I have a question about the GNAL antibody in question is NBP1-00840. Can you tell me what is known about this antibody's cross- reactivity with G alpha S? I am looking for an antibody that will detect G alpha olf specifically, and I note that the picture shows positive western blot in a human colon cancer cell line. Does the cell line express G alpha olf?
The cross-reactivity of NBP1-00840 and GNAS has not been evaluated. However, there is no homology between the immunogen for NBP1-00840 and the GNAS protein, therefore I do not expect there to be any interaction. In addition to our testing data, there is information in the literature about the expression of G alpha olf in colorectal cells as well as in protein databases. Please see Genecards and PubMed for additional information.