GM130/GOLGA2 Antibody

Images

 
Western Blot: GM130 Antibody [NB100-74430] - 50 ug of Hela cell lysate.
Immunocytochemistry/ Immunofluorescence: GM130 Antibody [NB100-74430] - Staining in p19 Cells.
Immunocytochemistry/ Immunofluorescence: GM130 Antibody [NB100-74430] - Staining in A549 Cells.
Immunocytochemistry/ Immunofluorescence: GM130 Antibody [NB100-74430] - Staining in HMVEC Cells.

Product Details

Summary
Product Discontinued
View other related GM130/GOLGA2 Primary Antibodies

Order Details


    • Catalog Number
      NB100-74430
    • Availability
      Product Discontinued

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GM130/GOLGA2 Antibody Summary

Immunogen
Synthetic peptide corresponding to residues G(368) Q V M E S V R Q L Q M E R D K(383) of human GM130.
Marker
Golgi Apparatus Marker
Specificity
GM130
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GOLGA2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
Application Notes
WB: Detects an approx. 135 kDa band representing GM130 from Hela cell lysate.
Publications
Read Publication using NB100-74430.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 1 mg/ml BSA
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GM130/GOLGA2 Antibody

  • 130 kDa cis-Golgi matrix protein
  • GM130 autoantigen
  • GM130
  • GM130Golgi matrix protein GM130
  • GOLGA2
  • golgi autoantigen, golgin subfamily a, 2
  • golgin A2
  • Golgin subfamily A member 2
  • Golgin-95
  • MGC20672
  • SY11 Protein

Background

The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. GM130 encodes a member of the Golgin protein family and is localized to the Golgi. The protein encoded by GM130 has been postulated to play roles in the stacking of Golgi cisternae, maintaining cis-Golgi structure and in vesicular transport. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined. GM130 is part of a larger oligomeric complex which interacts with p115, GORASP1/GRASP65 and RAB1B that has been activated by GTP-binding. GM130 is useful as a cis-Golgi marker in immunohistochemistry.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GM130/GOLGA2 Antibody (NB100-74430)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Diseases for GM130/GOLGA2 Antibody (NB100-74430)

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PTMs for GM130/GOLGA2 Antibody (NB100-74430)

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Bioinformatics

Gene Symbol GOLGA2
Entrez