Recombinant Human GM-CSF Protein

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-43192
    • Availability
      Product Discontinued

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Recombinant Human GM-CSF Protein Summary

Description
A partial protein corresponding to CSF2.
Preparation
Method
E. coli expressed amino acids ala 18 - glu144 of mature human GMCSF (accession # NM_000758).
Details of Functionality
This recombinant human GM-CSF has been tested in bioassays using human TF-1 cell line. The ED50 measured in a TF-1 proliferation assay is typically 100 pg/ml, corresponding to a specific activity of approximately 1.0 x10^7Units/mg.
Protein/Peptide Type
Partial Recombinant Protein
Gene
CSF2
Endotoxin Note
Less than 0.01 ng/ug cytokine as determined by the LAL assay.

Reactivity Notes

This protein was made to the human sequence.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
Sterile liquid; phosphate buffered saline, pH 7.2, 150 mM NaCl, with 1.0% BSA. 0.22um filtered.
Preservative
0.09% Sodium Azide

Alternate Names for Recombinant Human GM-CSF Protein

  • colony stimulating factor 2 (granulocyte-macrophage)
  • Colony-stimulating factor
  • CSF
  • CSF2
  • GMCSF
  • GM-CSF
  • GMCSFgranulocyte-macrophage colony-stimulating factor
  • granulocyte-macrophage colony stimulating factor
  • MGC131935
  • MGC138897
  • molgramostin
  • sargramostim

Background

Human GM-CSF (Granulocyte/Monocyte-Colony Stimulating Factor) is a differentially glycosylated factor produced mainly by activated T cells and macrophages. Endothelial cells and fibroblasts can also produce GM-CSF after exposure to TNF-alpha, IL-1, IL-2 and IFN-gamma. GM-CSF is found associated with extracellular matrix and in membrane-bound formats too. GM-CSF stimulates proliferation, activation and differentiation of macrophages and granulocytes and their progenitors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol CSF2
Entrez