Recombinant Mouse GM-CSF Protein

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-42897
    • Availability
      Product Discontinued

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Recombinant Mouse GM-CSF Protein Summary

Description
A partial protein corresponding to CSF2.
Preparation
Method
E. coli expressed mature mouse GM-CSF (accession # NM_009969).
Details of Functionality
The ED50 of this protein, as measured by MC/9 cell proliferation assay, is less than or equal to 650 pg/mL. This corresponds to a specific activity of greater than or equal to 1.5 x 10^6 Units/mg.
Protein/Peptide Type
Partial Recombinant Protein
Gene
CSF2
Endotoxin Note
Less than 0.01 ng/ug cytokine as determined by the LAL assay.

Applications/Dilutions

Application Notes
This reagent has been tested by sandwich ELISA using the anti-mouse GM-CSF antibody pair (MP1-22E9/MP1-31G6).

Reactivity Notes

This protein was made to the mouse sequence.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
Sterile liquid; phosphate buffered saline, pH 7.2, 150 mM NaCl, 0.22 um filtered.
Preservative
0.09% Sodium Azide

Notes

Purity: Greater than 98%, as determined by SDS-PAGE

Alternate Names for Recombinant Mouse GM-CSF Protein

  • colony stimulating factor 2 (granulocyte-macrophage)
  • Colony-stimulating factor
  • CSF
  • CSF2
  • GMCSF
  • GM-CSF
  • GMCSFgranulocyte-macrophage colony-stimulating factor
  • granulocyte-macrophage colony stimulating factor
  • MGC131935
  • MGC138897
  • molgramostin
  • sargramostim

Background

Mouse Granulocyte/Macrophage Colony Stimulating Factor (GM-CSF) is an ~14 kDa factor produced mainly by activated T cells and macrophages. Other cell types, such as endothelium and fibroblasts, also secrete GM-CSF in response to TNF-alpha, IL-2, IL-1, and IFN-gamma. GM-CSF stimulates growth of macrophages, granulocytes and dendritic cells. GM-CSF is found as a membrane-bound form and also as a complex associated with the extracellular matrix. Non-glycosylated GM-CSF is biologically active.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol CSF2
Entrez