GM-CSF Recombinant Protein


SDS-Page: GM-CSF Protein [NBC1-18455] - GM-CSF, 14.6 kDa (128 aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

Product Details

Reactivity HuSpecies Glossary
Applications PAGE
1.0 mg/ml

Order Details

GM-CSF Recombinant Protein Summary

A recombinant protein to GM-CSF.


Details of Functionality
The ED50 for this effect is < 0.18 ng/ml. Measured in a cell proliferation assay using TF1 human erythroleukemic cells.
Endotoxin Note
< 1.0 EU per 1 microgram of protein (determined by LAL method)

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
Liquid. In phosphate-buffered saline (PBS), pH 7.4
No Preservative
1.0 mg/ml


Application Notes
The purity of this protein is > 95% by SDS-PAGE. Molecular weight is 14.6 kDa (128 aa), confirmed by MALDI-TOF.

Cell line: TF1 (human erythroleukemic cell)
Maintenance Condition: 10% FBS RPMI 1640 with GM-CSF
Assay medium: 10% FBS RPMI 1640 without Gm-CSF
Cell density: 2 x 10,000 cells/well (96 well plate, final volume 100ul)
Starvation: 24hr, Assay medium
Incubation time: 24 hr (after sample treatment)
Concentration range: 0.048 ng/ml - 25 ng/ml
Detection method: MTT assay

Reactivity Notes


Alternate Names for GM-CSF Recombinant Protein

  • colony stimulating factor 2 (granulocyte-macrophage)
  • Colony-stimulating factor
  • CSF
  • CSF2
  • GM-CSF
  • GMCSFgranulocyte-macrophage colony-stimulating factor
  • granulocyte-macrophage colony stimulating factor
  • MGC131935
  • MGC138897
  • molgramostin
  • sargramostim


Human GM-CSF is a 14.6 kDa globular protein consisting of 128 amino acid containing two dislufied bonds. GM-CSF is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It is produced in by endothelial cells, monocytes, fibroblasts and T-lymphocytes. GM-CSF inhibits neutrophil migration and enhances the functional activity of the mature end-cells. Recombinant human GM-CSF was expressed in E.coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Contact Information

Product PDFs


Gene Symbol CSF2

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