Glycophorin A + B Antibody (HIR2) [PE]

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Flow Cytometry: Glycophorin A + B Antibody (HIR2) [PE] [NB500-587] - Surface staining of U937 human histiocytic lymphoma cell line with anti-human CD54 (1H4) PE. Total viable cells were used for analysis.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
HIR2
Clonality
Monoclonal
Host
Mouse
Conjugate
PE

Order Details

Glycophorin A B Antibody (HIR2) [PE] Summary

Immunogen
Raji cells and spleen cells fused with NS1 cells
Specificity
The antibody 1H4 reacts with CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein (receptor for rhinovirus) expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells; the expression of CD54 is upregulated by activation.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
GYPA
Purity
Size Exclusion Chromatography
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Flow Cytometry
Application Notes
The reagent is designed for Flow Cytometry analysis of human blood cells using 20 ul reagent / 100 ul of whole blood or 10^6 cells in a suspension.
Publications
Read Publication using NB500-587.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS and 0.2% (w/v) high grade BSA (protease free)
Preservative
15mM Sodium Azide
Purity
Size Exclusion Chromatography

Alternate Names for Glycophorin A B Antibody (HIR2) [PE]

  • CD235a antigen
  • glycophorin A (includes MN blood group)
  • glycophorin A (MNS blood group)
  • glycophorin Erik
  • glycophorin MiI
  • glycophorin MiIII
  • glycophorin MiV
  • glycophorin MiX
  • glycophorin SAT
  • glycophorin Sta type C
  • glycophorin-A
  • GPA
  • GPErik
  • GpMiIII
  • GPSAT
  • HGpMiIII
  • HGpMiV
  • HGpMiX
  • HGpMiXI
  • HGpSta(C)
  • Mi.V glycoprotein (24 AA)
  • MN sialoglycoprotein
  • MNS
  • PAS-2
  • recombinant glycophorin A-B Miltenberger-DR

Background

CD54 (ICAM-1) is a 90 kD member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol GYPA

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