Glutathione Peroxidase 1/GPX1 Antibody

Western Blot: Glutathione Peroxidase 1/GPX1 Antibody [NBP2-33429] - Analysis in human cell lines U-251MG and MCF-7 using anti-GPX1 antibody. Corresponding GPX1 RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.

Immunocytochemistry/Immunofluorescence: Glutathione Peroxidase 1/GPX1 Antibody [NBP2-33429] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: Glutathione Peroxidase 1/GPX1 Antibody [NBP2-33429] - Staining of human lymph node shows strong cytoplasmic positivity in subset of germinal center and non-germinal center cells.

Product Discontinued

Glutathione Peroxidase 1/GPX1 Antibody Summary

• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: RYSRRFQTIDIEPDIEALLSQGPSCA
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GPX1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Reactivity Notes

Rat (81%). Reactivity reported in scientific literature (PMID: 24743550)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Glutathione Peroxidase 1/GPX1 Antibody

• Cellular glutathione peroxidase
• CGPx
• EC 1.11.1
• EC 1.11.1.9
• Glutathione Peroxidase 1
• GPX1
• GPx-1
• GSHPX1
• GSHPx-1
• MGC14399
• MGC88245

Background

FUNCTION: Protects the hemoglobin in erythrocytes from oxidative breakdown. CATALYTIC ACTIVITY: 2 glutathione + H2O2 = glutathione disulfide + 2 H2O. SUBUNIT: Homotetramer. SUBCELLULAR LOCATION: Cytoplasm. SIMILARITY: Belongs to the glutathione peroxidase family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Glutathione Peroxidase 1/GPX1 Antibody (NBP2-33429)(2)

Publications using NBP2-33429

• Lee JR, Roh JL, Lee SM et al. Overexpression of glutathione peroxidase 1 predicts poor prognosis in oral squamous cell carcinoma J. Cancer Res. Clin. Oncol. 2017-06-26 [PMID: 28653098] (IHC-P, Human)
• Bachmann J, Burte F, Pramana S et al. Affinity Proteomics Reveals Elevated Muscle Proteins in Plasma of Children with Cerebral Malaria. PLoS Pathog 2014-04-01 [PMID: 24743550]

Bioinformatics

• Gene Symbol: GPX1
• Uniprot:
  • P07203()