Glutathione Peroxidase 1/GPX1 Antibody (13B2AF)

Western Blot: Glutathione Peroxidase 1/GPX1 Antibody (13B2AF) [NBP1-39565]

Immunohistochemistry-Paraffin: Glutathione Peroxidase 1/GPX1 Antibody (13B2AF) [NBP1-39565] - Analysis of anti-GPX1 antibody with human spleen at dilution 1:50.

Product Discontinued

Glutathione Peroxidase 1/GPX1 Antibody (13B2AF) Summary

• Immunogen: Recombinant mouse protein purified from E. coli (Glutathione Peroxidase 1/GPX1).
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: GPX1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:5000

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: HEPES, 0.01% BSA, 0.15M NaCl and 50% Glycerol
• Preservative: 0.03% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Glutathione Peroxidase 1/GPX1 Antibody (13B2AF)

• Cellular glutathione peroxidase
• CGPx
• EC 1.11.1
• EC 1.11.1.9
• Glutathione Peroxidase 1
• GPX1
• GPx-1
• GSHPX1
• GSHPx-1
• MGC14399
• MGC88245

Background

This gene encodes a member of the glutathione peroxidase family. Glutathione peroxidase functions in the detoxification of hydrogen peroxide, and is one of the most important antioxidant enzymes in humans. It has been reported that the protein encoded by this gene protects from CD95-induced apoptosis in cultured breast cancer cells and inhibits 5-lipoxygenase in blood cells, and its overexpression delays endothelial cell growth and increases resistance to toxic challenges. This protein is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by the nonsense (stop) codon TGA. In addition, this protein is characterized in a polyalanine sequence polymorphism in the N-terminal region, which includes three alleles with five, six or seven alanine (ALA) repeats in this sequence. The allele with five ALA repeats is significantly associated with breast cancer risk. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GPX1
• Entrez:
  • 2876(Human)
  • 14775(Mouse)
  • 817046(Rat)
• Uniprot:
  • P11352(Mouse)