Glutamine Synthetase Antibody (1F4)

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Western Blot: Glutamine Synthetase Antibody (1F4) [NBP2-02125] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-Glutamine Synthetase monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; ...read more
Immunocytochemistry/ Immunofluorescence: Glutamine Synthetase Antibody (1F4) [NBP2-02125] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Glutamine Synthetase.
Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody (1F4) [NBP2-02125] - Staining of paraffin-embedded Human Ovary tissue using anti-Glutamine Synthetase mouse monoclonal antibody.
Flow Cytometry: Glutamine Synthetase Antibody (1F4) [NBP2-02125] - Analysis of Jurkat cells, using anti-Glutamine Synthetase antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Western Blot: Glutamine Synthetase Antibody (1F4) [NBP2-02125] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Glutamine Synthetase (Right lane) cDNA for 48 hrs and lysed. ...read more
Immunocytochemistry/ Immunofluorescence: Glutamine Synthetase Antibody (1F4) [NBP2-02125] - Immunofluorescent staining of HepG2 cells using anti-Glutamine Synthetase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody (1F4) [NBP2-02125] - Staining of paraffin-embedded Human breast tissue using anti-Glutamine Synthetase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody (1F4) [NBP2-02125] - Staining of paraffin-embedded Human endometrium tissue using anti-Glutamine Synthetase mouse monoclonal antibody.
Flow Cytometry: Glutamine Synthetase Antibody (1F4) [NBP2-02125] - Analysis of Hela cells, using anti-Glutamine Synthetase antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, MkSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
1F4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Glutamine Synthetase Antibody (1F4) Summary

Immunogen
Full length human recombinant protein of human GLUL (NP_002056) produced in HEK293T cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
GLUL
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:50-100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Glutamine Synthetase Antibody (1F4)

  • cell proliferation-inducing protein 59
  • EC 4.1.1.15
  • EC 6.3.1.2
  • GLNSPIG59
  • Glutamate decarboxylase
  • glutamate-ammonia ligase (glutamine synthase)
  • glutamate-ammonia ligase
  • Glutamate--ammonia ligase
  • glutamine synthase
  • glutamine synthetase
  • GS
  • PIG43
  • proliferation-inducing protein 43

Background

The protein encoded by this gene belongs to the glutamine synthetase family. It catalyzes the synthesis of glutamine from glutamate and ammonia. Glutamine is a main source of energy and is involved in cell proliferation, inhibition of apoptosis, and cell signaling. This gene is expressed during early fetal stages, and plays an important role in controlling body pH by removing ammonia from circulation. Mutations in this gene are associated with congenital glutamine deficiency. Several alternatively spliced transcript variants have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol GLUL
Entrez