Glut4 Antibody

Images

 
Western Blot: Glut4 Antibody [NBP1-51262] - Extracts from A549 cells.
Immunohistochemistry-Paraffin: Glut4 Antibody [NBP1-51262] - Human lung carcinoma tissue.

Product Details

Summary
Product Discontinued
View other related Glut4 Primary Antibodies

Order Details


    • Catalog Number
      NBP1-51262
    • Availability
      Product Discontinued

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Glut4 Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 250-300 of Human Glut 4.
Clonality
Polyclonal
Host
Rabbit
Gene
SLC2A4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analyzes of Glut4 (R271) pAb in extracts from A549 cells.
Immunohistochemistry (IHC) analyzes of Glut4 (R271) pAb in paraffin-embedded human lung carcinoma tissue.
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP1-51262 in the following applications:

  • IHC
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Glut4 Antibody

  • Glut4
  • GLUT-4
  • GLUT4Glucose transporter type 4, insulin-responsive
  • insulin-responsive glucose transporter type 4
  • SLC2A4
  • solute carrier family 2 (facilitated glucose transporter), member 4
  • solute carrier family 2, facilitated glucose transporter member 4

Background

Glucose is fundamental to the metabolism of mammalian cells. Its passage across cell membranes is mediated by a family of transporters termed glucose transporters or Gluts. In adipose and muscle tissue, insulin stimulates a rapid and dramatic increase in glucose uptake, which is largely due to the redistribution of the insulin-inducible glucose transporter, Glut4. In response to insulin, Glut4 is quickly shuttled from an intracellular storage site to the plasma membrane, where it binds glucose. In contrast, the ubiquitously expressed glucose transporter Glut1 is constitutively targeted to the plasma membrane and shows a much less dramatic translocation in response to insulin. Glut1 and Glut4 are twelve pass transmembrane proteins (12TM) whose carboxy-termini may dictate their cellular localization. Aberrant Glut4 expression has been suggested to contribute to such maladies as obesity and diabetes. Glut4 null mice have shown that while functional Glut4 protein is not required for maintaining normal glucose levels, it is necessary for sustained growth, normal cellular glucose, fat metabolism and prolonged longevity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Glut4 Antibody (NBP1-51262)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IHC.


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IHC
(1)
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Mouse
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Glut4 Antibody (NBP1-51262)

Discover related pathways, diseases and genes to Glut4 Antibody (NBP1-51262). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for Glut4 Antibody (NBP1-51262)

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Blogs on Glut4.

Glucose Transporter 4 (GLUT4, SLC2A4)
GLUT4 is an insulin-sensitive glucose transporter that facilitates insulin-stimulated glucose uptake in adipose tissue, skeletal muscle, and cardiac tissues that specifically express this protein. It is a twelve transmembrane domain multi-pass protein...  Read full blog post.

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Bioinformatics

Gene Symbol SLC2A4
Entrez
Uniprot