Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS, approx. pH 7.2.
15mM Sodium Azide
Immunogen affinity purified
Western Blot 1:500-1:1000
Western blot (WB) analyzes of Glut4 (R271) pAb in extracts from A549 cells. Immunohistochemistry (IHC) analyzes of Glut4 (R271) pAb in paraffin-embedded human lung carcinoma tissue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publication using NBP1-51262 in the following applications:
GLUT4Glucose transporter type 4, insulin-responsive
insulin-responsive glucose transporter type 4
solute carrier family 2 (facilitated glucose transporter), member 4
solute carrier family 2, facilitated glucose transporter member 4
Glucose is fundamental to the metabolism of mammalian cells. Its passage across cell membranes is mediated by a family of transporters termed glucose transporters or Gluts. In adipose and muscle tissue, insulin stimulates a rapid and dramatic increase in glucose uptake, which is largely due to the redistribution of the insulin-inducible glucose transporter, Glut4. In response to insulin, Glut4 is quickly shuttled from an intracellular storage site to the plasma membrane, where it binds glucose. In contrast, the ubiquitously expressed glucose transporter Glut1 is constitutively targeted to the plasma membrane and shows a much less dramatic translocation in response to insulin. Glut1 and Glut4 are twelve pass transmembrane proteins (12TM) whose carboxy-termini may dictate their cellular localization. Aberrant Glut4 expression has been suggested to contribute to such maladies as obesity and diabetes. Glut4 null mice have shown that while functional Glut4 protein is not required for maintaining normal glucose levels, it is necessary for sustained growth, normal cellular glucose, fat metabolism and prolonged longevity.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Glucose Transporter 4 (GLUT4, SLC2A4) GLUT4 is an insulin-sensitive glucose transporter that facilitates insulin-stimulated glucose uptake in adipose tissue, skeletal muscle, and cardiac tissues that specifically express this protein. It is a twelve transmembrane domain multi-pass protein... Read full blog post.