Glut2 Antibody (5D1)

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Immunohistochemistry-Paraffin: Glut2 Antibody (5D1) [NBP1-40176] - Analysis of anti-GLUT2 antibody with human liver at concentration 10 ug/ml.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IHC-P, ICC
Clone
5D1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Glut2 Antibody (5D1) Summary

Immunogen
Human Glut2-transfected NS0 cells.
Specificity
Recognizes human GLUT 2 (Glucose Transporter, Insulin Regulatable) expression on hGlut2-transfected NS0 cells but not on control transfectants. Detects Glut2 on the surface of the human intestinal cell line Caco2 in flow cytometry and on fixed cells in immunocytochemistry tests. Based on flow cytometric tests on transfected cells, this antibody has no cross-reactivity with hGlut1 or hGlut3.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
SLC2A2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10 - 1:1000
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Immunocytochemistry 10 ug/ml

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 5% Trehalose
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for Glut2 Antibody (5D1)

  • GLUT2 Glucose transporter type 2, liver
  • Glut2
  • GLUT-2
  • SLC2A2
  • solute carrier family 2 (facilitated glucose transporter), member 2
  • solute carrier family 2, facilitated glucose transporter member 2

Background

Glucose transporters are integral membrane glycoproteins involved in transporting glucose into most cells. Seven types of glucose transport carrier proteins, designated as Glut 1 to 7, facilitate glucose transport across the cell membrane. Molecular cloning of glucose transporters have identified a family of closely related genes that encode at least 7 proteins exhibiting high degree of amino acid homology (45% to 65%), all in the molecular weight range of 40 to 60 kDa. Individual members of the Glut family have predicted secondary structure characteristic of 12 membrane spanning domains of other transport carriers. The majority of differences in sequence homology in Glut proteins occur at 4 hydrophilic domains that may play a role in distinct tissue specific pattern of expression and targeting. All Glut proteins are glycosylated at or near the C terminus and are present on either cell surface or in intracellular sites. Some transporters exhibit dynamic trafficking between intracellular storage sites and plasma membranes in response to various stimuli. In some tissues Glut proteins are asymmetrically distributed between apical and basolateral membranes as in blood brain barrier and blood testis barriers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for Glut2 Antibody (NBP1-40176). (Showing 1 - 1 of 1 FAQ).

  1. We are interested in using Glucose Transporter GLUT2 Antibody NBP1-40176 to test with rat samples. Could you please let me know if this antibody will react with the rat sequence?
    • This antibody was made against full length human GLUT2. However, the antibody is not epitope mapped so we do not know where along the sequence the antibody specifically binds. The full length sequence has 90% homology and 81% identity with the rat sequence, but as this has not been tested by our lab we cannot guarantee it to work with rat. If you are interested in testing this antibody in rat, you would become eligible for our Innovators Reward Program.

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Bioinformatics

Gene Symbol SLC2A2
Entrez
Uniprot