GluR2 Antibody [Unconjugated]

Images

 
Western blot of rat hippocampal lysate showing the specific immunolabeling of the approximately 100 kDa GluR2 protein.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ch, Pm, ZeSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

GluR2 Antibody [Unconjugated] Summary

Immunogen
GluR2
Specificity
This antibody is specific for the 100 kDa GluR2 in Western blots of rat brain lysates.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GRIA2
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000 dilution

Packaging, Storage & Formulations

Storage
For long-term storage, ≤ ‑20° C is recommended. Product is stable at ≤ ‑20° C for at least 1 year.
Buffer
100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.
Preservative
No Preservative

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GluR2 Antibody [Unconjugated]

  • AMPA 2
  • AMPA2
  • GluA2
  • GluR2
  • GLURB
  • GluR-K2
  • glutamate receptor, ionotropic, AMPA 2
  • GRIA2
  • HBGR2

Background

Rat GluR2 is a 100 kDa, 862 amino acid, 4-transmembrane (TM) glycoprotein that belongs to the ionotrophic/glutamate-gated ion channel family. Due to an impenetrate TM region 2, the N-terminus is extracellular and C-terminus intracellular. GluR2 is the most widely expressed of four AMPA receptor subunits, and contributes to the formation of a functional, presumably heterotetrameric glutamate receptor. AMPA receptors mediate fast excitatory transmission on postsynaptic membranes. The GluR2 subunit in particular regulates Ca, Zn and Na entry into the cell. It is unique among AMPA subunits in that it contains an Arginine in the second transmembrane segment (amino acid 607). This accounts for its ability to regulate ion flow. Upon neuronal insult, the Arginine can be converted to Glutamine, which allows for rapid Ca++ influx and the initiation of apoptosis. Alternatively, GluR2 levels may drop, allowing for multiple GluR combinations that allow rapid Ca++ influx. GluR2 is regulated both by palmitoylation and phosphorylation. Palmitoylation at C610 results in decreased surface expression. Phosphorylation by PKC on S880, and by Src on Y876, induces internalization. Human and rat GluR2 are 99% aa identical. Rat GluR3 is 866 aa in length and shares 72% overall aa identity with GluR2, with 85% aa identity over the C-terminal 15 amino acids. It is apparently not phosphorylated. GluR3 has a Ca++-permissive Glutamine at amino acid 612, the homolog to GluR2 R607. GluR3 is restricted in its expression pattern and is found on cerebellar Purkinje and Golgi Type II cells, neurons of the lateral amygdala, and cells of the basal ganglia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol GRIA2
Entrez