Recombinant E. coli Glucose 6 Phosphate Dehydrogenase Protein

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Western Blot: Glucose 6 Phosphate Dehydrogenase Protein [NBC1-19064] - 55.7kDa (491 aa) on SDS - PAGE

Product Details

Summary
Reactivity EcSpecies Glossary
Applications Func, PAGE
Concentration
1 mg/ml

Order Details

Recombinant E. coli Glucose 6 Phosphate Dehydrogenase Protein Summary

Description
An un-tagged recombinant protein corresponding to the amino acids1-491 of E.coli Glucose 6 Phosphate Dehydrogenase

Source: E.coli

Amino Acid Sequence: MAVTQTAQAC DLVIFGAKGD LARRKLLPSL YQLEKAGQLN PDTRIIGVGR ADWDKAAYTK VVREALETFM KETIDEGLWD TLSARLDFCN LDVNDTAAFS RLGAMLDQKN RITINYFAMP PSTFGAICKG LGEAKLNAKP ARVVMEKPLG TSLATSQEIN DQVGEYFEEC QVYRIDHYLG KETVLNLLAL RFANSLFVNN WDNRTIDHVE ITVAEEVGIE GRWGYFDKAG QMRDMIQNHL LQILCMIAMS PPSDLSADSI RDEKVKVLKS LRRIDRSNVR EKTVRGQYTA GFAQGKKVPG YLEEEGANKS SNTETFVAIR VDIDNWRWAG VPFYLRTGKR LPTKCSEVVV YFKTPELNLF KESWQDLPQN KLTIRLQPDE GVDIQVLNKV PGLDHKHNLQ ITKLDLSYSE TFNQTHLADA YERLLLETMR GIQALFVRRD EVEEAWKWVD SITEAWAMDN DAPKPYQAGT WGPVASVAMI TRDGRSWNEF E

Details of Functionality
Specific activity is > 70 units/mg obtained by measuring the increase of beta-NADPH in absorbance at 340 nm resulting from the reduction of beta-NADP. One unit oxidizes 1.0 umole D-glucose-6-phosphate to 6-phospho-D-gluconate per min in the presence of beta-NADP at pH 7.4 at 25C.
Source
E. coli
Protein/Peptide Type
Biologically Active Protein
Gene
G6PD
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Theoretical MW
55.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
50 mM MES 6.0, 0.1 mM PMSF, 2 mM EDTA, 0.5 mM DTT, 10% glycerol
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant E. coli Glucose 6 Phosphate Dehydrogenase Protein

  • G6PD
  • G6PD1EC 1.1.1.49
  • glucose-6-phosphate dehydrogenase

Background

Glucose 6 Phosphate Dehydrogenase (G6PD) is the rate-limiting enzyme of the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells by maintaining the level of NADPH. G6PD converts glucose-6-phosphate into 6-phosphoglucono-delta-lactone and simultaneously produce NADPH. The NADPH in turn maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. G6PD deficiency cause acute hemolytic anemia. Recombinant G6PD protein was expressed in E.coli and purified by conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

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Bioinformatics

Gene Symbol G6PD