GFP Antibody

Analysis of GFP in transgenic mouse pancreas in OCT. The blue region in the center is an islet which does not express GFP and is negative. Image from verified customer review.

Analysis of GFP tagged PKA subunits over-expressed in HEK293T cells using GFP antibody. Image from verified customer review.

Staining of GFP+ hepatocytes (brown) transplanted into the liver of a recipient animal using anti-GFP antibody. Cyclin D1 double staining in blue. Image from verified customer review.

Total cell lysates (20ug) with protein of interest tagged with GFP was loaded. Blotted with GFP antibody [HRP] NB600-308H, without any secondary antibody. Image from verified customer review.

Germaria from wt or chico1/KG mutant ovaries endogenously expressing Myc-GFP stained with anti-GFP, anti-1B1, and DAPI. Scale bars, 10 um. (F) Quantification of relative Myc-GFP intensity in germarium region 2B from wt or chico1/KG mutant ovaries. n = 10 germaria for each genotype. Error bars represent SEM. *p<0.005. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31612862/) licensed under a CC-BY licence.

Western Blot of GFP antibody. Lane 1: Wild type GFP (0.1 ug) was used to spike HeLa whole cell lysate. Lane 2: none. Load: 30 ug per lane.Primary antibody: GFP antibody at 1:1000 for overnight at 4C.Secondary antibody: IRDye800(TM) Goat-a-Rabbit IgG [H&L] MX10 at 1:10,000 for 45 min at RT.Block: 5% BLOTTO in PBS overnight at 4C.Predicted/Observed size: 27 kDa for epitope tag GFP. Other band(s): none.

Western Blot of GFP antibody. Lane 1: 293FT cells transfected with CDK4 dominant negative (C-).Lane 2: 293FT cells positive control (C+).Load: 25 ug per lane.Primary antibody: GFP antibody at 1:400 for overnight at 4C.Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT.Block: 5% BLOTTO overnight at 4C.Predicted/Observed size: 27 kDa for GFP.

Western Blot of GFP antibody. Marker: Opal Pre-stained ladder. Lane 1: HEK293 lysate. Lane 2: HeLa Lysate. Lane 3: CHO/K1 Lysate. Lane 4: MDA-MB-231. Lane 5: A431 Lysate. Lane 6: Jurkat Lysate. Lane 7: NIH/3T3 Lysate. Lane 8: E-coli HCP Control. Lane 9: FLAG Positive Control Lysate. Lane 10: Red Fluorescent Protein. Lane 11: Green Fluorescent Protein. Lane 12: Glutathione-S-Transferase Protein. Lane 13: Maltose Binding Protein. Load: 10 ug of lysate or 50ng of purified protein per lane. Primary antibody: GFP antibody at 1ug/mL overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:30,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS for 30 min at RT.Predicted/Observed size: 30 kDa for GFP.

Adult mouse brain tissue. This image was submitted via customer review.

Immuno-microscopy of GFP antibody. Monocyte derived dendritic cells and dermal macrophages were challenged and directly visualized with eGFP labeled Dengue virus to localize sequestration of virus particles in the different cells (upper). The location of the GFP was confirmed by TEM (lower magnified view) using rabbit anti GFP Primary antibody (1:200) and a gold labeled secondary antibody.

Analysis of GFP in mouse retinal cells. Image courtesy of anonymous customer review.

IP analysis of GFP in S2 Cells (Drosophila). Image from verified customer review.

Immunoprecipitation experiments showing that GFP-MISP binds endogenous IQGAP1 (upper panel) and MISP binds to IQGAP1 endogenously using specific antibodies (lower panel) in mitotically arrested (taxol and nocodazole, respectively) HeLa cell lysates. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-24682-8), licensed under a CC-BY licence.

• Reactivity: All-NA
• Applications: WB, DB, ELISA, Flow, ICC/IF, IHC, IP, MS, IHC, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

GFP Antibody Summary

• Description: This antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria)
  
  GFP antibody is stable for several weeks at 4C as an undiluted liquid.
• Immunogen: The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
• Specificity: No reaction was observed against Human, Mouse or Rat serum proteins.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Dot Blot
  • Electron Microscopy 1:10-1:500
  • ELISA 1:20000-1:120000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:500-1:5000
  • Immunohistochemistry Free-Floating
  • Immunohistochemistry Whole-Mount
  • Immunohistochemistry 1:200-1:3000
  • Immunohistochemistry-Frozen 1:50-1:250
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Knockdown Validated
  • Western Blot 1:500-1:5000
• Application Notes: This product is designed to detect GFP and its variants. GFP antibody has been tested by western blot and ELISA. This product can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. Biotin conjugated polyclonal anti-GFP used in a sandwich ELISA is well suited to titrate GFP in solution using this antibody in combination with monoclonal anti-GFP using either form of the antibody as the capture or detection antibodies. However, use the monoclonal form only for the detection of wild type or recombinant GFP as this form does not sufficiently detect 'enhanced' GFP. The detection antibody is typically conjugated to biotin and subsequently reacted with streptavidin conjugated HRP. Fluorochrome conjugated polyclonal anti-GFP can be used to detect GFP by immunofluorescence microscopy in prokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and can detect GFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-GFP relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-GFP to detect GFP or GFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher.
  
  Use in Immunoprecipitation reported in scientific literature (PMID:34887587).
  
  Use in DB reported in scientific literature (PMID:34242364).
  
  Use in Knockdown Validated reported in scientific literature
  (PMID: 32905777).

Reactivity Notes

No reaction was observed against Human, Mouse serum proteins. Suitable for detecting fusion proteins containing the GFP sequence expressed in Human, Mouse, Rat, C. elegans, Drosophila and in vitro transcription/translation systems and transgenic animals. Known cross reactivity with wt and all variants such as rGFP, eGFP, S65T-GFP, RS-GFP, YFP and EGFP.

GFP Transgenic Rat reactivity reported in scientific literature (PMID:25724725).

Mouse reactivity reported in multiple pieces of scientific literature.

Transgenic C. elegans reactivity reported in scientific literature (PMID: 27110099).

Use in Mouse reported in secitific publication (PMID: 32765228).

Plant reactivity reported in scientific literature (PMID:32896843)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for GFP Antibody

• anti-gfp (green fluorescent protein) pab
• eGFP
• Enhanced Green Fluorescent Protein
• GFP immunofluorescence
• GFP immunoprecipitation
• GFP mouse
• GFP staining
• GFP western blot
• GFP
• GFPuv
• green fluorescent protein (gfp)
• Green Fluorescent Protein

Background

Green fluorescent protein (GFP) is a 27 kDa protein derived from the bioluminescent jellyfish Aquorea Victoria. GFP can be excited by light in the blue to ultraviolet spectrum and has an excitation peak at 395 nm with an emission peak at 509 nm in the green spectrum. GFP autocatalytically forms a fluorescent pigment from natural amino acids present in the nascent protein. GFP is widely used as a reporter (tag) for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. GFP fluorescence is stable under fixation conditions and antibodies are suitable for a variety of applications including ELISA, ICC/IF, IHC, IP and WB (1-3).

References
1. Shi, C., Pan, F. C., Kim, J. N., Washington, M. K., Padmanabhan, C., Meyer, C. T., . . . Means, A. L. (2019). Differential Cell Susceptibilities to Kras(G12D) in the Setting of Obstructive Chronic Pancreatitis. Cell Mol Gastroenterol Hepatol. doi:10.1016/j.jcmgh.2019.07.001

2. Zhao, S., Fortier, T. M., & Baehrecke, E. H. (2018). Autophagy Promotes Tumor-like Stem Cell Niche Occupancy. Curr Biol, 28(19), 3056-3064.e3053. doi:10.1016/j.cub.2018.07.075

3. Zusso, M., Lunardi, V., Franceschini, D., Pagetta, A., Lo, R., Stifani, S., . . . Moro, S. (2019). Ciprofloxacin and levofloxacin attenuate microglia inflammatory response via TLR4/NF-kB pathway. J Neuroinflammation, 16(1), 148. doi:10.1186/s12974-019-1538-9

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GFP Antibody (NB600-308)(235)

Publications using NB600-308

• Xing L, Simon JM, Ptacek TS et al. Autism-linked UBE3A gain-of-function mutation causes interneuron and behavioral phenotypes when inherited maternally or paternally in mice Cell reports 2023-06-28 [PMID: 37389991] (WB)
  
  Details:
  WB 1:2000
• Samuel T, Rapic S, O'Brien C et al. Quantitative intravital imaging for real-time monitoring of pancreatic tumor cell hypoxia and stroma in an orthotopic mouse model Science advances 2023-06-09 [PMID: 37285434] (WB)
  
  Details:
  1:50 WB dilution
• Bao K, Isik Can U, Miller MM et al. A bifurcated role for c-Maf in Th2 and Tfh2 cells during helminth infection Mucosal immunology 2023-04-21 [PMID: 37088263] (IHC)
• Xing D, Hage FG, Feng W et al. Endothelial Cells Overexpressing CXCR1/2 are Renoprotective in Rats with Acute Kidney Injury American journal of physiology. Renal physiology 2023-02-16 [PMID: 36794755] (IHC-P, Rat)
• Boffa I, Polishchuk E, De Stefano L et al. Liver-directed gene therapy for ornithine aminotransferase deficiency EMBO molecular medicine 2023-04-11 [PMID: 36647689] (WB)
• Obara K, Shirai K, Hamada Y et al. Direct implantation of hair-follicle-associated pluripotent (HAP) stem cells repairs intracerebral hemorrhage and reduces neuroinflammation in mouse model PloS one 2023-01-13 [PMID: 36638123] (IHC-P, Mouse)
  
  Details:
  Dilution used in IHC-P 1:1000
• Stupin A Atg1 modulates mitochondrial dynamics to promote germline stem cell maintenance in Drosophila Biochemical and Biophysical Research Communications 2022-11-01 [PMID: 36621115] (IF/IHC)
  
  Details:
  Drosophila expressing GFP
• Ghochani Y, Muthukrishnan SD, Sohrabi A et al. A molecular interactome of the glioblastoma perivascular niche reveals integrin binding sialoprotein as a mediator of tumor cell migration Cell reports 2022-10-18 [PMID: 36261010]
• Zhang W, Lanzoni G, Hani H et al. Patch Grafting of Cells into Solid Organs Such as Liver and Pancreas Biomaterials 2022-08-27 [PMID: 36030102]
• Rourke C, Jaramillo-Lambert A TOP-2 is differentially required for the proper maintenance of the cohesin subunit REC-8 on meiotic chromosomes in C. elegans spermatogenesis and oogenesis Genetics 2022-08-11 [PMID: 35951744] (IHC-Fr, Human)
  
  Details:
  IHC-Fr 1:500
• Nam YW, Pala R, El-Sayed NS et al. Subtype-Selective Positive Modulation of KCa2.3 Channels Increases Cilia Length ACS chemical biology 2022-08-19 [PMID: 35947779] (WB, Mouse)
• Ma JH, Lee E, Yoon SH et al. Therapeutic effect of NLRP3 inhibition on hearing loss induced by systemic inflammation in a CAPS-associated mouse model EBioMedicine 2022-07-20 [PMID: 35870427] (IHC-Fr)
• Hanna DN, Smith PM, Novitskiy SV et al. SMAD4 suppresses colitis-associated carcinoma through inhibition of CCL20/CCR6-mediated inflammation Gastroenterology 2022-07-18 [PMID: 35863523]
• Osaki Y, Manolopoulou M, Ivanova AV et al. Blocking cell cycle progression through CDK4/6 protects against chronic kidney disease JCI insight 2022-06-22 [PMID: 35730565] (FLOW, IF/IHC)
• Feng C, Wang Y, Zha X et al. Cold-sensitive ventromedial hypothalamic neurons control homeostatic thermogenesis and social interaction-associated hyperthermia Cell metabolism 2022-06-07 [PMID: 35675799] (IF/IHC)
• Ma L, Chen C, Liu D et al. Apoptotic extracellular vesicles are metabolized regulators nurturing the skin and hair Bioactive materials 2023-01-01 [PMID: 35600968] (ICC/IF)
• Law SSY, Liou G, Nagai Y et al. Polymer-coated carbon nanotube hybrids with functional peptides for gene delivery into plant mitochondria Nature communications 2022-05-16 [PMID: 35577779] (WB)
• Gu T, Hao D, Woo J et al. The disordered N-terminal domain of DNMT3A recognizes H2AK119ub and is required for postnatal development Nature genetics 2022-05-01 [PMID: 35534561] (WB)
• Lao M, Hurtado A, de Castro AC et al. Sox9 is required for nail bed differentiation and digit tip regeneration The Journal of investigative dermatology 2022-04-07 [PMID: 35398376]
• Thagun C, Horii y, Mori M et al. Non-transgenic Gene Modulation via Spray Delivery of Nucleic Acid/Peptide Complexes into Plant Nuclei and Chloroplasts ACS nano 2022-02-23 [PMID: 35195009] (WB)
• Kakoty V, C SK, Yang CH et al. Neuroprotective Effect of Lentivirus-Mediated FGF21 Gene Delivery in Experimental Alzheimer's Disease is Augmented when Concerted with Rapamycin Molecular neurobiology 2022-02-10 [PMID: 35142986]
• Elbaz M, Callado Perez A, Demers M et al. A vibrissa pathway that activates the limbic system eLife 2022-02-10 [PMID: 35142608] (IF/IHC)
• Sun J, Yoon J, Lee M et al. Zic5 stabilizes Gli3 via a non-transcriptional mechanism during retinal development Cell reports 2022-02-01 [PMID: 35108539] (ICC/IF)
• Osokine I, Siewiera J, Rideaux D et al. Gene silencing by EZH2 suppresses TGF-beta activity within the decidua to avert pregnancy-adverse wound healing at the maternal-fetal interface Cell reports 2022-02-01 [PMID: 35108527]
• Chalif JI, MartInez-Silva ML, Pagiazitis JG et al. Control of mammalian locomotion by ventral spinocerebellar tract neurons Cell 2022-01-20 [PMID: 35063074] (IHC-P)
• Wu Y, Devotta A, JosE-Edwards DS et al. Xbp1 and Brachyury establish an evolutionarily conserved subcircuit of the notochord gene regulatory network eLife 2022-01-20 [PMID: 35049502] (ISH)
• Autry AE, Wu Z, Kohl J, Bambah-Mukku D Perifornical Area Urocortin-3 Neurons Promote Infant-directed Neglect and Aggression Elife 2021-08-23 [PMID: 34423776]
• Green EL Nicotinamide, neural cells, and Parkinsons disease: exploring nicotinamides effects on proliferation, neuronal differentiation, and neuroprotection within in vitro models Thesis 2021-01-01
• Chen B, Scurrah CR, McKinley ET et al. Differential pre-malignant programs and microenvironment chart distinct paths to malignancy in human colorectal polyps Cell 2021-12-22 [PMID: 34910928] (FLOW)
• Ramos H, Monette A, Niu M et al. The double-stranded RNA-binding protein, Staufen1, is an IRES-transacting factor regulating HIV-1 cap-independent translation initiation Nucleic acids research 2021-12-06 [PMID: 34893869] (WB)
• Shu XE, Mao Y, Jia L, Qian SB Dynamic eIF3a O-GlcNAcylation controls translation reinitiation during nutrient stress Nature chemical biology 2021-12-09 [PMID: 34887587] (IP)
• Joshi B, Wagh G, Kaur H, Patra C Zebrafish Model to Study Angiotensin II-Mediated Pathophysiology Biology 2021-11-13 [PMID: 34827169] (IF/IHC)
• McGuire MR, Mukhopadhyay D, Myers SL Et al. Progesterone receptor membrane component 1 (PGRMC1) binds and stabilizes cytochromes P450 through a heme-independent mechanism The Journal of biological chemistry 2021-10-19 [PMID: 34678314] (WB)
• Hausmann A, Felmy B, Kunz L et al. Intercrypt sentinel macrophages tune antibacterial NF-kappa B responses in gut epithelial cells via TNF J Exp Med 2021-11-01 [PMID: 34529751] (ICC/IF)
  
  Details:
  Citation using the Alexa Fluor 647 format of this antibody.
• Zhang W, Lanzoni G, Hani H Et al. Patch grafting, strategies for transplantation of organoids into solid organs such as liver Biomaterials 2021-08-01 [PMID: 34517276] (ICC/IF)
• Kong JH, Young CB, Pusapati GV Et al. Gene-teratogen interactions influence the penetrance of birth defects by altering Hedgehog signaling strength Development (Cambridge, England) 2021-10-01 [PMID: 34486668]
• Girish N, Liu CY, Gadeock S et al. Persistence of Lgr5+ colonic epithelial stem cells in mouse models of inflammatory bowel disease American journal of physiology. Gastrointestinal and liver physiology 2021-07-14 [PMID: 34260310] (IHC-P)
• Bourgeois Js, Wang L, Rabino Af Et Al. ARHGEF26 enhances Salmonella invasion and inflammation in cells and mice PLoS pathogens 2021-07-01 [PMID: 34242364] (Cytometric Bead Assay Standard)
• Singh B, Khattab F, Chae H et al. KATP channel blockers control glucagon secretion by distinct mechanisms: The direct stimulation of alpha-cells involving a [Ca2+]c rise and an indirect inhibition mediated by somatostatin Molecular metabolism 2021-06-09 [PMID: 34118477] (IF/IHC)
• Xu PF, Borges RM, Fillatre J et al. Construction of a mammalian embryo model from stem cells organized by a morphogen signalling centre Nature communications 2021-06-02 [PMID: 34078907] (IF/IHC)
• Bota-Rabassedas N, Banerjee P, Niu Y et al. Contextual cues from cancer cells govern cancer-associated fibroblast heterogeneity Cell reports 2021-04-20 [PMID: 33882319]
• McElvain LE, Chen Y, Moore JD et al. Specific populations of basal ganglia output neurons target distinct brain stem areas while collateralizing throughout the diencephalon Neuron 2021-04-01 [PMID: 33823137]
• Maniates KA, Olson BS, Abbott AL Sperm fate is promoted by the mir-44 microRNA family in the Caenorhabditis elegans hermaphrodite germline Genetics 2021-03-08 [PMID: 33683352]
• Yu D, G Febbo I, J Maroteaux M et al. The Transcription Factor Shox2 Shapes Neuron Firing Properties and Suppresses Seizures by Regulation of Key Ion Channels in Thalamocortical Neurons Cerebral cortex (New York, N.Y. : 1991) 2021-03-02 [PMID: 33675359] (IF/IHC, WB)
• Oikawa K, Imai T, Thagun C et al. Mitochondrial movement during its association with chloroplasts in Arabidopsis thaliana Communications biology 2021-03-05 [PMID: 33674706] (WB)
• Tang C, Han J, Dalvi S et al. A human model of Batten disease shows role of CLN3 in phagocytosis at the photoreceptor-RPE interface Commun Biol 2021-02-05 [PMID: 33547385] (WB)
• Becker R Regulation of non-centrosomal microtubule organizing center formation in skeletal muscle cells Thesis 2022-01-01
• González-Prieto R, Eifler-Olivi K, Claessens LA, et al. Global non-covalent SUMO interaction networks reveal SUMO-dependent stabilization of the non-homologous end joining complex Cell reports 2021-01-26 [PMID: 33503430]
• Bland KM, Aharon A, Widener EL, et al. FMRP regulates the subcellular distribution of cortical dendritic spine density in a non-cell-autonomous manner Neurobiology of disease 2021-01-06 [PMID: 33421563]
• Morales L, Castro-Robles B, AbellAn A et al. A novel telencephalon-opto-hypothalamic morphogenetic domain produces most of the glutamatergic neurons of the medial extended amygdala J Comp Neurol 2021-01-02 [PMID: 33386618]
• Vergarajauregui S, Becker R, Steffen U et al. AKAP6 orchestrates the nuclear envelope microtubule-organizing center by linking golgi and nucleus via AKAP9 eLife 2020-12-09 [PMID: 33295871] (Non-species specific)
• Wolter JM, Mao H, Fragola G et al. Cas9 gene therapy for Angelman syndrome traps Ube3a-ATS long non-coding RNA Nature 2020-10-21 [PMID: 33087932]
• Sade N, Weng F, Tajima H et al. A Cytoplasmic Receptor-like Kinase Contributes to Salinity Tolerance Plants (Basel) 2020-10-17 [PMID: 33080797]
• Im JY, Kim DM, Park H et al. Bacterial Lipoteichoic Acid Attenuates Toll-Like Receptor Dependent Dendritic Cells Activation and Inflammatory Response Biochim Biophys Acta Mol Cell Res 2020-10-08 [PMID: 33069758] (WB)
• Hamaoui, D, CossE, M M Et al. The Chlamydia effector CT622/TaiP targets a nonautophagy related function of ATG16L1. Proc Natl Acad Sci U S A 2020-10-27 [PMID: 33055216] (IF/IHC)
• Donato C, Kunz L, Castro-Giner F et al. Hypoxia Triggers the Intravasation of Clustered Circulating Tumor Cells Cell reports 2020-09-08 [PMID: 32905777] (KD, ICC/IF, WB)
• Thagun C , Motoda Y , Kigawa T et al. Simultaneous introduction of multiple biomacromolecules into plant cells using a cell-penetrating peptide nanocarrier Nanoscale 2020-09-28 [PMID: 32896843] (WB)
• Kim H, Berens NC, Ochandarena NE, Philpot BD Region and Cell Type Distribution of TCF4 in the Postnatal Mouse Brain Front Neuroanat 2020-07-17 [PMID: 32765228] (ICC/IF)
• Saha C, Mohanraju P, Stubbs A et al. Guide-free Cas9 from pathogenic Campylobacter jejuni bacteria causes severe damage to DNA Sci Adv 2020-06-01 [PMID: 32596446] (WB)
• Cuijpers SAG, Willemstein E, Ruppert JG et al. Chromokinesin KIF4A teams up with Stathmin 1 to regulate abscission in a SUMO-dependent manner J. Cell. Sci. 2020-06-26 [PMID: 32591481] (WB)
• Frantz MG, Crouse EC, Sokhadze G et al. Layer 4 Gates Plasticity in Visual Cortex Independent of a Canonical Microcircuit Curr. Biol. 2020-06-18 [PMID: 32589913]
• Shin E Y, Park J H et al. Integrin-mediated adhesions in regulation of cellular senescence. Sci Adv 2020-01-05 [PMID: 32494696] (WB, IF/IHC)
• Min H, Youn E, Shim YH Maternal Caffeine Intake Disrupts Eggshell Integrity and Retards Larval Development by Reducing Yolk Production in a Caenorhabditis elegans Model Nutrients 2020-05-07 [PMID: 32392893] (WB)
• Chen Z, Wang ZH, Zhang G et al. Mitochondrial DNA segregation and replication restrict the transmission of detrimental mutation J. Cell Biol. 2020-07-06 [PMID: 32375181] (ICC/IF)
• Taniguchi T, Zanetti-Yabur A, Wang P et al. Interindividual Diversity in Expression of Organic Anion Uptake Transporters in Normal and Cirrhotic Human Liver Hepatol Commun 2020-05-05 [PMID: 32363323] (WB)
• Monette A, Niu M, Chen L et al. Pan-retroviral Nucleocapsid-Mediated Phase Separation Regulates Genomic RNA Positioning and Trafficking Cell Rep 2020-04-23 [PMID: 32320662] (WB)
• Steinberg EE, Gore F, Heifets BD et al. Amygdala-Midbrain Connections Modulate Appetitive and Aversive Learning Neuron 2020-04-16 [PMID: 32294466]
• Kashiwagi H, Ishimoto H, Izumi SI et al. Human PZP and common marmoset A2ML1 as pregnancy related proteins Sci Rep 2020-03-22 [PMID: 32198464] (IF/IHC)
• Hu X, Deng Q, Ma L et al. Meningeal lymphatic vessels regulate brain tumor drainage and immunity Cell Res 2020-02-26 [PMID: 32094452] (IHC-Fr)
• Tsukamoto T, Gearhart M D et al. Insights into the Involvement of Spliceosomal Mutations in Myelodysplastic Disorders from Analysis of SACY-1/DDX41 in Caenorhabditis elegans. Genetics 2020-01-04 [PMID: 32060018] (WB)
• Corbet C, Bastien E, Santiago de Jesus JP et al. TGFbeta2-induced formation of lipid droplets supports acidosis-driven EMT and the metastatic spreading of cancer cells Nat Commun 2020-01-23 [PMID: 31974393] (IF/IHC)
• Dubey R, Stivala CE, Nguyen HQ et al. Lipid droplets can promote drug accumulation and activation Nat. Chem. Biol. 2020-02-01 [PMID: 31932720] (WB)
• Thagun C, Chuah JA, Numata K. Targeted Gene Delivery into Various Plastids Mediated by Clustered Cell-Penetrating and Chloroplast-Targeting Peptides Adv Sci (Weinh) 2019-12-14 [PMID: 31832328] (WB, IP)
• Kim B K, Cheong J H et al. PI3K/AKT/beta-Catenin Signaling Regulates Vestigial-Like 1 Which Predicts Poor Prognosis and Enhances Malignant Phenotype in Gastric Cancer. Cancers (Basel) 2019-03-12 [PMID: 31816819] (WB)
• Kunz L, Schroeder T A 3D Tissue-wide Digital Imaging Pipeline for Quantitation of Secreted Molecules Shows Absence of CXCL12 Gradients in Bone Marrow Cell Stem Cell 2019-12-05 [PMID: 31809740] (ICC/IF)
• Wang Z H, Liu Y et al. Electron transport chain biogenesis activated by a JNK-insulin-Myc relay primes mitochondrial inheritance in Drosophila. Elife 2019-10-15 [PMID: 31612862] (IF/IHC)
• Mercer Lindsay N, Knutsen PM, Lozada AF et al. Orofacial Movements Involve Parallel Corticobulbar Projections from Motor Cortex to Trigeminal Premotor Nuclei Neuron 2019-09-20 [PMID: 31587918]
• Gewin L, Summers ME, Harral JW et al. Inactivation of Tsc2 in Abcg2 Lineage Derived Cells Drives the Appearance of Polycystic Lesions and Fibrosis in the Adult Kidney Am. J. Physiol. Renal Physiol. 2019-08-28 [PMID: 31461347]
• Guo B, Itami J et al. Native protein delivery into rice callus using ionic complexes of protein and cell-penetrating peptides. PLoS One 2019-07-31 [PMID: 31361745] (WB)
• Zusso M, Lunardi V, Franceschini D et al. Ciprofloxacin and levofloxacin attenuate microglia inflammatory response via TLR4/NF-kB pathway J Neuroinflammation 2019-07-18 [PMID: 31319868] (WB)
• Shi C, Pan FC, Kim JN et al. Differential Cell Susceptibilities to Kras(G12D) in the Setting of Obstructive Chronic Pancreatitis Cell Mol Gastroenterol Hepatol 2019-01-01 [PMID: 31310834] (IF/IHC)
• English JG, Olsen RHJ, Lansu K et al. VEGAS as a Platform for Facile Directed Evolution in Mammalian Cells Cell 2019-07-25 [PMID: 31280962]
• Lee M, Hwang Y S et al. Developmentally regulated GTP-binding protein 1 modulates ciliogenesis via an interaction with Dishevelled. J Cell Biol 2019-03-07 [PMID: 31270137] (IP, IF/IHC)
  
  Details:
  Xenopus laevis (African clawed frog)
• Nilangekar K, Murmu N, Sahu G et al. Generation and Characterization of Germline-Specific Autophagy and Mitochondrial Reactive Oxygen Species Reporters in Drosophila Front Cell Dev Biol 2019-04-20 [PMID: 31001531] (WB)
• Cruz-Ortega JS, Boucard AA. Actin cytoskeleton remodeling defines a distinct cellular function for adhesion G protein-coupled receptors ADGRL/latrophilins 1, 2 and 3 Biol Open 2019-03-31 [PMID: 30926595] (WB)
• Sandhu R, Wei D, Sharma M et al. An N-terminal Flag-tag impairs TPP1 regulation of telomerase function Biochem Biophys Res Commun 2019-03-20 [PMID: 30885434] (WB)
• Li A, Cho JH, Reid B et al. Calcium oscillations coordinate feather mesenchymal cell movement by SHH dependent modulation of gap junction networks Nat Commun 2018-12-19 [PMID: 30560870] (IF/IHC)
• Di Scala C, Tessier M, Sapet C et al. A new polymer-based approach for in vivo transfection in postnatal brain. J Neurosci Methods 2019-01-01 [PMID: 30408559]
• Li Q, Saito TT, Martinez-Garcia M et al. The tumor suppressor BRCA1-BARD1 complex localizes to the synaptonemal complex and regulates recombination under meiotic dysfunction in Caenorhabditis elegans PLoS Genet 2018-11-02 [PMID: 30383767] (WB)
• Zhao S, Fortier TM, Baehrecke EH. Autophagy Promotes Tumor-like Stem Cell Niche Occupancy Curr Biol 2018-10-02 [PMID: 30270184] (ICC/IF)
• Cain NE, Jahed Z, Schoenhofen A et al. Conserved SUN-KASH Interfaces Mediate LINC Complex-Dependent Nuclear Movement and Positioning Curr Biol 2018-09-25 [PMID: 30245107] (ICC/IF)
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Reviews for GFP Antibody (NB600-308) (18)

Average Rating: 4.5

(Based on 18 reviews)

We have 18 reviews tested in 4 species: Human, Mouse, Rat, Other.

reviewed by: Abhijit Nirwane IHC-Fr Mouse 04/12/2018

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Adult mouse brain tissue
• Species: Mouse
• Lot: 35459

Comments

• Comments: Dilutions 1:400

reviewed by: Priyanka Manandhar ICC Mouse 03/22/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: FFPE
• Species: Mouse

Comments

• Comments: Trypsin retrieval

reviewed by: Verified Customer WB Human 03/10/2017

Summary

• Application: Western Blot
• Sample Tested: whole cellular protein and fractionated (soluble and membrane protein)
• Species: Human

Comments

• Comments: Human embryonic kidney 293T
  HEK293T cells

reviewed by: Verified Customer Immunofluorescence on tissues. Human 03/02/2017

Summary

• Application: Other
• Sample Tested: Human Xenograft Tumor
• Species: Human

reviewed by: Anonymous - IF Other 07/27/2016

Summary

• Application: Immunofluorescence
• Sample Tested: Pig TM cells
• Species: Other

reviewed by: Ganesh Pusapati IF Mouse 10/10/2015

Summary

• Application: Immunofluorescence
• Sample Tested: Mouse, 3T3 cells
• Species: Mouse
• Lot: 28839

reviewed by: Ganesh Pusapati WB Human 10/10/2015

Summary

• Application: Western Blot
• Sample Tested: HEK293T whole cell extract
• Species: Human
• Lot: 28839

reviewed by: Marcio Fontenele IP Other 04/02/2015

Summary

• Application: Immunoprecipitation
• Sample Tested: S2 Cells (Drosophila)
• Species: Other

reviewed by: Verified Customer IF Mouse 11/25/2014

Summary

• Application: Immunofluorescence
• Sample Tested: transgenic mouse pancreas
• Species: Mouse

reviewed by: Fabio Marongiu IHC-P 10/05/2014

Summary

• Application: Immunohistochemistry-Paraffin
• Lot: 28839

reviewed by: Verified Customer IHC-Fr Mouse 11/06/2013

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Transgenic mouse pancreas in OCT
• Species: Mouse
• Lot: 24437

reviewed by: Verified Customer IF Rat 07/13/2012

Summary

• Application: Immunofluorescence
• Sample Tested: Rat liver
• Species: Rat
• Lot: 24437

reviewed by: Verified Customer IP Human 04/24/2012

Summary

• Application: Immunoprecipitation
• Sample Tested: Hela whole cell lysate
• Species: Human

reviewed by: Verified Customer WB Other 11/15/2011

Summary

• Application: Western Blot
• Sample Tested: Yeast
• Species: Other

reviewed by: Anna Means IHC Mouse 09/28/2011

Summary

• Application: Immunohistochemistry
• Sample Tested: IHC on parrafin sections (mouse)
• Species: Mouse

reviewed by: Verified Customer IF Mouse 07/20/2011

Summary

• Application: Immunofluorescence
• Sample Tested: Mouse Retinal Cells
• Species: Mouse

reviewed by: Verified Customer WB Other 11/23/2010

Summary

• Application: Western Blot
• Sample Tested: Pig ear fibroblasts, Sample Amount: 10 ul
• Species: Other

reviewed by: Verified Customer WB Mouse 09/23/2010

Summary

• Application: Western Blot
• Sample Tested: P19 cells transfectedwith a plasmid DNA expressing a GFP fusion protein, Sample Amount: 20 micrograms
• Species: Mouse

FAQs for GFP Antibody (NB600-308). (Showing 1 - 2 of 2 FAQs).

1. Does the GFP antibody NB600-308 also work against AcGFP (93% identical to EGFP) in IP and Western blot?
   • For our product NB600-308, we have unfortunately not yet tested the product for cross reactivity with AcGFP. However, since the antibody is polyclonal and made to the full length AvGFP sequence, there is a good chance for cross reactivity.
2. Hello, my lab purchased a GFP antibody from Novus in the past and I am now in need of an antibody to detect YFP and CFP as well. The GFP antibody we order has a part number of NB600-308. Would it possibly recognize YPF and CFP as well or will I need to purchase additional antibodies?
   • NB600-308 is polyclonal to full length GFP, hence it will recognize most of its mutants. It will react with YFP and CFP.

Bioinformatics

• Uniprot:
  • P42212()