GFP Antibody

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Western Blot: GFP Antibody [NB100-56402] - Analysis of extracts from 293A cells, unstransfected (lane 1) and transfected (lane 2) with GFP expression vector, using GFP antibody (NB100-56402) at 1:1000 (0.5ug/ml).
Western Blot: GFP Antibody [NB100-56402] - Analysis of Green Fluorescent Protein in 20 ugs of A) transfected HeLa cells and B) untransfected HeLa cells using Green Fluorescent Protein antibody at 0.5 ug/ml.

Product Details

Summary
Reactivity NASpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

GFP Antibody Summary

Immunogen
Amino acids 204-217 of Green Fluorescent Protein were used as immunogen.
Specificity
The Green Fluorescent Protein amino acid sequence used as immunogen for this antibody is 100% homologous in YFP (Yellow) and CFP (Cyan).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-2 ug/ml
Publications
Read Publications using
NB100-56402 in the following applications:

  • WB
    1 publication

Reactivity Notes

Chemical

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for GFP Antibody

  • eGFP
  • Enhanced Green Fluorescent Protein
  • GFP
  • GFPuv
  • green fluorescent protein (gfp)
  • Green Fluorescent Protein

Background

Green Fluorescent Protein (GFP) is often used as a reporter molecule for gene expression. Cotransfecting Green Fluorescent Protein with a gene of interest allows researchers to rapidly determine the success of a transfection.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Publications for GFP Antibody (NB100-56402)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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WB
(1)
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Filter By Species
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for GFP Antibody (NB100-56402). (Showing 1 - of FAQs).

    Secondary Antibodies

     

    Isotype Controls

    Additional Array Products

    Array NB100-56402

    Research Areas for GFP Antibody (NB100-56402)

    Find related products by research area.

    Blogs on GFP.

    The use of a GFP antibody for research applications in transgenic C. elegans, GFP tagged yeast and porcine model
    GFP, or green fluorescent protein, is a chemiluminescent protein derived from Aequorea jellyfish that was first discovered by Osamu Shimomura.  It was soon after established that the emission spectra of GFP was right around 509nm, or the ultraviol...  Read full blog post.

    GFP - Be Green!
    Green fluorescence protein (GFP) is a 27KD protein derived from the jellyfish Aquorea victoria that emits a green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP is a highly v...  Read full blog post.

    GFP (Green Fluorescent Protein) Bringing Light to Life
    GFP (green florescent protein), originally discovered in the jellyfish Aequorea victoria, (1) is one of the most extensively investigated and exploited proteins in the area of life sciences. GFP is well known for its proficiency emit fluorescence and...  Read full blog post.

    BDNF Antibodies Aid Research on Alzheimer's Therapies
    Brain-derived neurotrophic factor (BDNF) is known to be important for neuronal differentiation, survival, migration and plasticity in both the developing embryo and adult synapses. The BDNF antibody is also proving to be an important tool in Alzheimer...  Read full blog post.

    Epitope Tags and the V5 Tag Antibody
    We at Novus Biologicals have a large antibody catalog targeted to epitope tags. They include C-Myc, GFP, HA, DYKDDDDK, and V5 Tag antibody reagents. The V5 tag antibody recognizes the artificially engineered V5 epitope, which is formed of amino acid r...  Read full blog post.

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