GFP Antibody (BV-F4)

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Western Blot: GFP Antibody (BV-F4) [NBP1-50696] - GFP expressed in 293 cells transfected with a GFP-fusion construct.

Product Details

Summary
Product Discontinued
View other related GFP Primary Antibodies

Order Details


    • Catalog Number
      NBP1-50696
    • Availability
      Product Discontinued

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GFP Antibody (BV-F4) Summary

Immunogen
Recombinant EGFP expressed from E. col
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
GFP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
Western blotting (0.5-4 ug/ml), immunoprecipitation (2-8 ug/ml), immunofluorescence (10-20 ug/ml) and Immunohistochemistry (10-20 ug/ml). However, the optimal conditions should be determined individually. The antibody recognizes wild-type GFP and its derivatives such as EGFP, EBFP, ECFP, and EYFP, etc. Performs well in Western blot analysis (0.5 to 4 ug/ml), immunoprecipitation, and Immunohistochemistry. However, the optimum conditions should be determined individually.

Reactivity Notes

The polyclonal antibody recognizes GFP, EGFP, EYFP fusion proteins in all species.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2), 0.5% BSA and 30% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GFP Antibody (BV-F4)

  • eGFP
  • Enhanced Green Fluorescent Protein
  • GFP
  • GFPuv
  • green fluorescent protein (gfp)
  • Green Fluorescent Protein

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for GFP Antibody (NBP1-50696). (Showing 1 - of FAQ).

Secondary Antibodies

 

Isotype Controls

Additional GFP Products

Bioinformatics Tool for GFP Antibody (NBP1-50696)

Discover related pathways, diseases and genes to GFP Antibody (NBP1-50696). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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GFP, or green fluorescent protein, is a chemiluminescent protein derived from Aequorea jellyfish that was first discovered by Osamu Shimomura.  It was soon after established that the emission spectra of GFP was right around 509nm, or the ultraviol...  Read full blog post.

GFP - Be Green!
Green fluorescence protein (GFP) is a 27KD protein derived from the jellyfish Aquorea victoria that emits a green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP is a highly v...  Read full blog post.

GFP (Green Fluorescent Protein) Bringing Light to Life
GFP (green florescent protein), originally discovered in the jellyfish Aequorea victoria, (1) is one of the most extensively investigated and exploited proteins in the area of life sciences. GFP is well known for its proficiency emit fluorescence and...  Read full blog post.

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Bioinformatics

Gene Symbol GFP
Entrez
Uniprot