GFAP Antibody (GA5) [HRP]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ch, RbSpecies Glossary
Applications WB, ELISA, IHC-P
Clone
GA5
Clonality
Monoclonal
Host
Mouse
Conjugate
HRP

Order Details

GFAP Antibody (GA5) [HRP] Summary

Immunogen
Glial fibrillary protein from porcine spinal cord was used as the immunogen for the GFAP (GA5) antibody.
Localization
Cytoplasmic
Marker
Astrocyte Marker
Specificity
This MAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
GFAP
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-2ug/ml
  • ELISA
  • Immunohistochemistry-Paraffin 0.25-0.5ug/ml
Readout System
Control
GFAP Knockout HeLa Cell Lysate

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
No Preservative
Purity
Protein G purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for GFAP Antibody (GA5) [HRP]

  • FLJ45472
  • GFAP astrocytes
  • GFAP
  • glial fibrillary acidic protein

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for GFAP Antibody (NBP2-33184H). (Showing 1 - 1 of 1 FAQ).

  1. I am interested in native or recombinant GFAP protein without GST Tag or in urea buffer. Can I use your GFAP antibody (NB100-53809) to detect P0809, P0810 and P1691 GFAP proteins?
    • P0809, P0810 and P1691 are blocking peptides that are not suitable for use with NB100-53809. Unfortunately, we do not carry a GFAP protein without a GST tag. I apologize for the inconvenience.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on GFAP. Showing 1-10 of 11 blog posts - Show all blog posts.

Deriving neural precursor cells from human induced pluripotent stem cells
By Jennifer Sokolowski, MD, PhD.Human induced pluripotent stem cells (iPSCs) can be used to create models of human organ systems and are useful for a) ascertaining the mechanisms underlying pathological conditions...  Read full blog post.

Identifying tumoral and stromal transcriptomes that underlie tumor plasticity and stromal neuroinflammatory response in brain metastasis
By Jamshed Arslan, Pharm. D., PhD. Cancers in the brain often come from tumors elsewhere in the body. Several adaptive mechanisms influence brain metastasis, such as blood brain barrier leakage that can be induced by...  Read full blog post.

Beta Tubulin III and neurogenesis
Beta tubulin III, also known as Tuj-1, is a class III member of the beta tubulin protein family. Beta tubulins are one of two structural components that form our microtubule network. While general tubulins play a role in a wide range of cellular pr...  Read full blog post.

The identification of dopaminergic neurons using Tyrosine Hydroxylase in Parkinson's research and LRRK2
Tyrosine hydroxylase (TH) is a crucial enzyme involved in the biosynthesis of dopamine, norepinephrine and epinephrine in the brain.  Specifically, TH catalyzes the conversion of l-tyrosine to l-dihydroxyphenylalanine (l-dopa).  The importance of t...  Read full blog post.

Synapsin I: Implicated in synaptic activity across a diverse range of studies
Synapsins are a family of neuronal proteins that are most renowned for their activity in modulating the pre-synaptic terminal.  Synapsin’s behavior is regulated by protein kinases and phosphatases, which alter the way that synapsin’s i...  Read full blog post.

Glial Fibrillary Acidic Protein (GFAP), The Most Popular Astrocyte Marker
GFAP, a class-III intermediate filament, is a 50kDa protein which is found in the mature and developing astrocytes in the CNS, non-myelinating Schwann cells in the PNS, enteric glial cells (enteric nervous system/ENS), ependymal cells, and radial g...  Read full blog post.

Nestin: Investigating the Link Between New Brain Cells and Depression
Clinical depression (also known as major depressive disorder or MDD) affects many people, but the biological processes that cause it (and are influenced by its treatments) are not well understood. Adult neurogenesis is a newly emerging field that coul...  Read full blog post.

GFAP: Roles in Alzheimer's and Schizophrenia
Glial fibrillary acidic protein (GFAP) is a class III intermediate filament (IF) protein and is used as a marker to distinguish astrocytes from other glial cells during development. GFP may play a role in maintaining mechanical strength and shape in a...  Read full blog post.

Vimentin in Wound Healing
Vimentin is a fundamental 10 nm type III intermediate filament (IF) protein found in many mesenchymal and epithelia tissues, tissue culture cells, and developing neuronal and astrocytic precursor cells of the central nervous system. It frequently co-p...  Read full blog post.

"Whats the Hap" with GFAP?
Glial Fibrillary Acidic Protein (GFAP) is one of the major intermediate filament axonal proteins found in mature astrocytes, the star-shaped glial cells that comprise the majority of cells within the central nervous system (1). Astrocytes perform a wi...  Read full blog post.

Showing 1-10 of 11 blog posts - Show all blog posts.

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Bioinformatics

Gene Symbol GFAP