GEF-H1 Antibody (B4/7)



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GEF-H1 Antibody (B4/7) Summary

Monoclonal antibody B4/7 recognizes canine guanine nucleotide exchange factor H1 (GEF-H1).
Protein A or G purified
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  • Western Blot 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
This product works in Immunofluorescence, Immunohistochemistry-Frozen, Immunoprecipitation and Western Blot.
Positive Control
GEF-H1 Lysate (NBP2-65348)

Reactivity Notes

Human, Canine

Packaging, Storage & Formulations

Store at 4C. Do not freeze.
0.2 um filtered solution in PBS and 0.1% BSA
0.02% Sodium Azide
Protein A or G purified

Alternate Names for GEF-H1 Antibody (B4/7)

  • GEF
  • GEFH1
  • GEF-H1DKFZp547P1516
  • Guanine nucleotide exchange factor H1
  • KIAA0651DKFZp547L106
  • LFP40FLJ45440
  • Microtubule-regulated Rho-GEF
  • P40
  • Proliferating cell nucleolar antigen p40
  • rho guanine nucleotide exchange factor 2
  • Rho/Rac guanine nucleotide exchange factor (GEF) 2
  • Rho/Rac guanine nucleotide exchange factor 2


Monoclonal antibody B4/7 recognizes canine guanine nucleotide exchange factor H1 (GEF-H1). GEF-H1 is an ~110 kDa protein belonging to the Dbl family of proto-oncogenes. GEF-H1 can activate the small GTPase RhoA, but not Rac1 or Cdc42. Rho family GTPases are central regulators of the cytoskeleton. GEF-H1 can associate with different cytoskeletal structures, namely microtubules and the actin cytoskeleton. It has also been proposed to mediate crosstalk between the two types of filaments. Localization of GEF-H1 differs between cell types. In MRC-5 fibroblast cells, GEF-H1 localizes to stress fibers. In epithelial cells, GEF-H1 is associated with apical tight junctions and involved in regulating paracellular permeability of small hydrophilic tracers. Furthermore, its subcellular localization changes in mitotic cells, where endogenous GEF-H1 is concentrated at mitotic spindles. GEF-H1 is capable of binding to the F-actin binding junctional adaptor cingulin. Binding to cingulin inhibits GEF-H1 and results in the downregulation of RhoA and inhibition of G1/S phase transition. In low confluent cultured cells, the localization of GEF-H1 is predominantly cytoplasmic. With increasing density of the cells, free GEF-H1 is sequestered at the tight junctions by cingulin. The monoclonal antibody B4/7 is raised against a protein fraction isolated from detergent extracts of MDCK cells. The monoclonal antibody B4/7 also recognizes human GEF-H1.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Positive Control Lysate(s)

Secondary Antibodies


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Gene Symbol ARHGEF2