GDF-6/BMP-13 Overexpression Lysate (Native)

Images

 
Western Blot: GDF6 Lysate [NBL1-11028] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for GDF6

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

GDF-6/BMP-13 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC211366 and based on accession number NM_001001557. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens growth differentiation factor 6 (GDF6), mRNA.
Gene
GDF6

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
48.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for GDF-6/BMP-13

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for GDF-6/BMP-13 Overexpression Lysate (Native)

  • BMP13
  • BMP-13
  • CDMP2
  • GDF16
  • GDF6
  • GDF-6
  • growth differentiation factor 6
  • Growth/differentiation factor 16
  • growth/differentiation factor 6
  • KFS
  • KFS1
  • KFSL
  • MCOP4
  • MCOPCB6
  • MGC158100
  • MGC158101
  • SCDO4
  • SGM1

Background

This gene encodes a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily of secreted signaling molecules. It is required for normal formation of some bones and joints in the limbs, skull, and axial skeleton. Mutations in this gene result in colobomata, which are congenital abnormalities in ocular development, and in Klippel-Feil syndrome (KFS), which is a congenital disorder of spinal segmentation. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for GDF-6/BMP-13 Lysate (NBL1-11028) (0)

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Video Protocols

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Bioinformatics

Gene Symbol GDF6
Entrez