GDF-15 Antibody (6D12.H10.E4) - C-terminus - BSA Free

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Genetic Strategies: Western Blot: GDF-15 Antibody (6D12.H10.E4) [NBP2-44214] - Western blot shows lysates of HepG2 human liver carcinoma parental cell line and GDF-15 knockout (KO) HepG2 cell line. PVDF membrane ...read more
Western Blot: GDF-15 Antibody (6D12.H10.E4) [NBP2-44214] - Analysis of Rat Anti-Nag 1 (C terminal specific). Lane 1: Protein Standard Opal Pre-stained. Lane 2: MBP-NAG-1 fusion protein 1. Lane 3: MBP-NAG-1 fusion ...read more
Western Blot of GDF-15 Antibody (6D12.H10.E4) - C-terminus. Lane 1: Opal Prestained Molecular Weight Marker

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, KD
Clone
6D12.H10.E4
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Format
BSA Free
Validated by:
     

Genetic Strategies

   

Order Details

GDF-15 Antibody (6D12.H10.E4) - C-terminus - BSA Free Summary

Description
This product was purified from concentrated tissue culture supernatant Protein G chromatography

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Immunogen
This Protein-A purified GDF-15 Antibody (6D12.H10.E4) was prepared by repeated immunizations with an MBP-tagged recombinant protein produced in E.coli corresponding to C-term mouse NAG-1 protein. Cross reactivity to MBP is negative. (Uniprot: Q99988)
Specificity
This antibody reacts with the C-terminus of endogenous GDF-15 protein from mouse tissues. A BLAST analysis suggests reactivity with GDF-15 from chimpanzee and macaque based on a 100% homology. Partial reactivity is expected against rat based on an 86% homology with the immunizing sequence. Cross-reactivity with GDF-15 from other sources has not been determined.
Isotype
IgG2a Lambda
Clonality
Monoclonal
Host
Rat
Gene
GDF15
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA 1:2000
  • Knockdown Validated
  • Western Blot 1:1000
Application Notes
This Protein G This product has been tested by ELISA, SDS-PAGE, and western blot for mouse NAG-1 protein. Specific conditions for reactivity should be optimized by the end user. Expect bands in Western blots of native protein of approximately ~58kDa using the appropriate cell lysate or extract.
Publications
Read Publication using NBP2-44214.

Reactivity Notes

A BLAST analysis suggests reactivity with GDF-15 from chimpanzee and macaque based on a 100% homology. Partial reactivity is expected against rat based on an 86% homology with the immunizing sequence. Cross-reactivity with GDF-15 from other sources has not been determined.

Packaging, Storage & Formulations

Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Protein G purified

Alternate Names for GDF-15 Antibody (6D12.H10.E4) - C-terminus - BSA Free

  • GDF15
  • GDF-15
  • growth differentiation factor 15
  • growth/differentiation factor 15
  • Macrophage inhibitory cytokine 1
  • MIC-1
  • MIC-1NSAID-activated gene 1 protein
  • MIC1Prostate differentiation factor
  • NAG-1
  • NAG-1NSAID-regulated gene 1 protein
  • NSAID (nonsteroidal inflammatory drug)-activated protein 1
  • PDF
  • PDFGDF-15
  • PLAB
  • PLABNRG-1
  • Placental bone morphogenetic protein
  • Placental TGF-beta
  • PTGF-beta
  • PTGFBPTGF-beta

Background

Growth differentiation factor 15 (GDF15) is a transforming growth factor beta superfamily member that acts to regulate inflammatory and apoptotic responses in injured or diseased tissues. GDF15 is most strongly expressed in the liver, placenta and kidney.

GDF15 antibodies are useful for studies on inflamation and immune system research.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GDF-15 Antibody (NBP2-44214)(1)

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Secondary Antibodies

 

Isotype Controls

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Jamshed Arslan, Pharm D, PhD Tissues within the human body are made of a three-dimensional (3D) arrangement of cells working together to perform vital functions. The commonly used 2D monolayer cultures have limited ...  Read full blog post.

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Bioinformatics

Gene Symbol GDF15
Uniprot