Recombinant Human GCSH His Protein

SDS-Page: Recombinant Human GCSH Protein [NBP1-72520] - 15% SDS-PAGE (3ug)

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human GCSH His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 49-173 of Human GCSH
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMSVRKFT EKHEWVTTEN GIGTVGISNF AQEALGDVVY CSLPEVGTKL NKQDEFGALE SVKAASELYS PLSGEVTEIN EALAENPGLV NKSCYEDGWL IKMTLSNPSE LDELMSEEAY EKYIKSIEE

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: GCSH
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 16.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, 1 mM DTT, 0.15 M NaCl
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human GCSH His Protein

• GCE
• glycine cleavage system H protein, mitochondrial
• glycine cleavage system protein H (aminomethyl carrier)
• lipoic acid-containing protein
• mitochondrial glycine cleavage system H-protein
• NKH

Background

The enzyme system for cleavage of glycine (glycine cleavage system) is composed of four mitochondrial protein components: P protein, H protein, T protein, and L protein. GCSH is the H protein, which transfers the methylamine group of glycine from the P protein to the T protein. Defects in this gene are a cause of nonketotic hyperglycinemia (NKH). Recombinant human GCSH protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: GCSH