GCP4 Antibody

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Western Blot: GCP4 Antibody [NBP1-00846] - Western blot (WB) analysis of GCP4 (L225) antibody in extracts from COLO cell.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-00846
    • Availability
      Product Discontinued

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GCP4 Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 197-243 of Human GCP4.
Specificity
GCP4 (L225) detects endogenous levels of GCP4 protein.
Clonality
Polyclonal
Host
Rabbit
Gene
TUBGCP4
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
Application Notes
Western blot (WB) analysis of GCP4 (L225) pAb in extracts from COLO cell.
Theoretical MW
76 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

This antibody is reactive against Human, Mouse

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GCP4 Antibody

  • 76PhGCP4
  • FLJ14797
  • gamma tubulin ring complex protein (76p gene)
  • Gamma-ring complex protein 76 kDa
  • gamma-tubulin complex component 4
  • GCP-4
  • GCP4hGrip76
  • h76p
  • tubulin, gamma complex associated protein 4

Background

The gamma-Tubulin complex is composed of gamma Tubulin and the gamma-Tubulin complexassociated proteins GCP2, GCP3, GCP4, GCP5 and GCP6, all of which are essential components of microtubule organizing centers. gamma-Tubulin complex components are localized to both the centrosome, where they are involved in microtubule nucleation, and to the cytoplasm, where they exist as soluble complexes that can be recruited to the centrosome as needed. Although the GCP proteins are related, they have distinct roles which contribute to the proper function of the gamma-Tubulin complex. GCP4 (gamma-Tubulin complex component 4), also known as TUBGCP4, is a ubiquitously expressed 667 amino acid member of the gamma-Tubulin complex that localizes to the metaphase spindle during mitosis. In response to proteosome inhibition, GCP4 exhibits increased accumulation at the pericentiolar material where it participates in microtubule organization and nucleation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol TUBGCP4
Entrez