GCLM Antibody

Images

 
Western Blot: GCLM Antibody [NBP2-16624] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with GCLM antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody ...read more
Immunocytochemistry/ Immunofluorescence: GCLM Antibody [NBP2-16624] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.
Western Blot: GCLM Antibody [NBP2-16624] - Sample (30 ug of whole cell lysate) A: A431 12% SDS PAGE gel, diluted at 1:1000.
Western Blot: GCLM Antibody [NBP2-16624] - Sample (50 ug of whole cell lysate) A: rat liver, 12% SDS PAGE gel, diluted at 1:1000.
Genetic Strategies: Western Blot: GCLM Antibody [NBP2-16624] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with GCLM ...read more
Western Blot: GCLM Antibody [NBP2-16624] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with GCLM antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

GCLM Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human GCLM. The exact sequence is proprietary.
Predicted Species
Bovine (96%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GCLM
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:10000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
Theoretical MW
31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for GCLM Antibody

  • Gamma-ECS regulatory subunit
  • Gamma-glutamylcysteine synthetase regulatory subunit
  • GCS light chain
  • GLCLRglutamate--cysteine ligase regulatory subunit
  • glutamate-cysteine ligase (gamma-glutamylcysteine synthetase), regulatory(30.8kD)
  • Glutamate--cysteine ligase modifier subunit
  • glutamate-cysteine ligase regulatory protein
  • glutamate-cysteine ligase, modifier subunit
  • GSC light chain

Background

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol GCLM
Uniprot