GBL Knockout HeLa Cell Lysate

Images

 
There are currently no images for GBL Lysate (NBP2-64757).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Concentration
LYOPH

Order Details

GBL Knockout HeLa Cell Lysate Summary

Preparation
Method
Knockout achieved by using CRISPR/Cas9,5 bp deletion in exon 2 and 1 bp insertion in exon 2
Gene
MLST8

Applications/Dilutions

Application Notes
You will receive 1 vial (100ug) of knockout cell lysate and 1 vial (100ug) of Parental cell lysate. Lysate can be diluted with 1X SDS sample buffer and will be stable at -20 degrees C for 12 months. Minimize freeze-thaw cycles.

Packaging, Storage & Formulations

Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Buffer
0.1 mg cell homogenate lyophilized in RIPA buffer made with double-knockout cell lines.
Concentration
LYOPH
Reconstitution Instructions
To use as WB negative control, spin down briefly and resuspend in 100 uL 1xSDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). Boil the lysate for 3 - 5 minutes before loading it onto gel.

Lysate Details for Array

Type
Knockout HeLa Cell

Notes

Powered by EDIGENE.
Validation of antibody specificity is critical and verification of antibody performance against knockout samples is one way to guarantee that an antibody recognizes a specific target. Novus' KO cell lysate can be used as a negative control for western blots and to confirm the specificity of antibodies.

Alternate Names for GBL Knockout HeLa Cell Lysate

  • G protein beta subunit-like
  • gable
  • GbetaL
  • GBL
  • GBLPop3
  • Lst8
  • LST8MGC111011
  • Mammalian lethal with SEC13 protein 8
  • mLST8
  • MTOR associated protein, LST8 homolog (S. cerevisiae)
  • POP3
  • Protein GbetaL
  • target of rapamycin complex subunit LST8
  • TORC subunit LST8
  • WAT1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB600-607
Species: Hu, Rt
Applications: WB, ELISA, IHC, IHC-P
NB100-331
Species: Hu, Mu, Rt
Applications: WB
AF887
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, CyTOF-ready, ICFlow
NB100-612
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
NB110-40424
Species: Hu, Mu
Applications: WB, IHC, IHC-P, IP
NBP2-24503
Species: Hu, Mu, Pm
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-22356
Species: Hu, Mu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NB200-157
Species: Hu
Applications: WB, IHC, IHC-P, KO
NBP1-89865
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
AF8962
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, CyTOF-ready, ICFlow
NBP2-67627
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
MAB40401
Species: Hu, Mu
Applications: WB, IHC, ICC
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP, PAGE
NB300-109
Species: Hu, Mu, Rt, Dr, I, Ma
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P, ICC, IHC-FrFl
MAB6890
Species: Hu, Mu
Applications: WB, ICC
NB100-1595
Species: Hu, Mu
Applications: WB, IHC, IHC-P, IP
H00006009-M01
Species: Hu, Mu, Rt
Applications: WB, ELISA
NBP1-87799
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P

Publications for GBL Lysate (NBP2-64757) (0)

There are no publications for GBL Lysate (NBP2-64757).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for GBL Lysate (NBP2-64757) (0)

There are no reviews for GBL Lysate (NBP2-64757). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for GBL Lysate (NBP2-64757) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Array Products

Bioinformatics Tool for GBL Lysate (NBP2-64757)

Discover related pathways, diseases and genes to GBL Lysate (NBP2-64757). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for GBL Lysate (NBP2-64757)

Discover more about diseases related to GBL Lysate (NBP2-64757).
 

Pathways for GBL Lysate (NBP2-64757)

View related products by pathway.

PTMs for GBL Lysate (NBP2-64757)

Learn more about PTMs related to GBL Lysate (NBP2-64757).
 

Research Areas for GBL Lysate (NBP2-64757)

Find related products by research area.

Blogs on GBL

There are no specific blogs for GBL, but you can read our latest blog posts.

Customers Who Bought This Also Bought

4EBP1 Antibody
NB200-157

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our GBL Knockout HeLa Cell Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol MLST8
Entrez