GBL Antibody


Western Blot: GBL Antibody [NBP2-16562] - Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE gel, diluted at 1:1000.

Product Details

Reactivity HuSpecies Glossary
Applications WB
1 mg/ml

Order Details

GBL Antibody Summary

Recombinant fragment corresponding to a region within amino acids 102 and 326 of G protein beta subunit like (Uniprot ID#Q9BVC4)
Predicted Species
Mouse (97%), Rat (97%), Bovine (99%)
Immunogen affinity purified
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Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
1XPBS, 1%BSA, 20% Glycerol (pH7).
0.01% Thimerosal
1 mg/ml
Immunogen affinity purified


Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
36 kDa

Reactivity Notes

Human. Expected to cross react based on sequence identity: Mouse (97%), Rat (97%), Bovine (99%)

Alternate Names for GBL Antibody

  • G protein beta subunit-like
  • gable
  • GbetaL
  • GBLPop3
  • Lst8
  • LST8MGC111011
  • Mammalian lethal with SEC13 protein 8
  • mLST8
  • MTOR associated protein, LST8 homolog (S. cerevisiae)
  • POP3
  • Protein GbetaL
  • target of rapamycin complex subunit LST8
  • TORC subunit LST8
  • WAT1


Subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Amino-acid-signaling to mTORC1 is mediated by Rag GTPases, which cause amino-acid-induced relocalization of mTOR within the endomembrane system. Growth factor-stimulated mTORC1 activation involves AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, LST8 interacts directly with FRAP1 and enhances its kinase activity. In nutrient-poor conditions, stabilizes the FRAP1-RPTOR interaction and favors RPTOR-mediated inhibition of FRAP1 activity. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Secondary Antibodies


Isotype Controls

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Contact Information

Product PDFs


Gene Symbol MLST8

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