GBE1 Antibody (1D11)

Images

 
Western Blot: GBE1 Antibody (1D11) [NBP2-02245] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-GBE1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: ...read more
Immunocytochemistry/ Immunofluorescence: GBE1 Antibody (1D11) [NBP2-02245] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY GBE1.
Immunohistochemistry-Paraffin: GBE1 Antibody (1D11) [NBP2-02245] - Staining of paraffin-embedded Human Ovary tissue using anti-GBE1 mouse monoclonal antibody.
Flow Cytometry: GBE1 Antibody (1D11) [NBP2-02245] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GBE1 antibody, and then analyzed ...read more
Western Blot: GBE1 Antibody (1D11) [NBP2-02245] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GBE1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ...read more
Immunocytochemistry/ Immunofluorescence: GBE1 Antibody (1D11) [NBP2-02245] - Immunofluorescent staining of HepG2 cells using anti-GBE1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GBE1 Antibody (1D11) [NBP2-02245] - Staining of paraffin-embedded Human colon tissue using anti-GBE1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GBE1 Antibody (1D11) [NBP2-02245] - Staining of paraffin-embedded Human endometrium tissue using anti-GBE1 mouse monoclonal antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, IP
Clone
1D11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

GBE1 Antibody (1D11) Summary

Immunogen
Full length human recombinant protein of human GBE1 (NP_000149) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
GBE1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:50-100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Immunoprecipitation 2ug/500ul

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for GBE1 Antibody (1D11)

  • 1,4-alpha-glucan-branching enzyme
  • amylo-(1,4 to 1,6) transglucosidase
  • amylo-(1,4 to 1,6) transglycosylase
  • Brancher enzyme
  • EC 2.4.1.18
  • GBE
  • glucan (1,4-alpha), branching enzyme 1
  • glycogen branching enzyme
  • Glycogen-branching enzyme

Background

The protein encoded by this gene is a glycogen branching enzyme that catalyzes the transfer of alpha-1,4-linked glucosyl units from the outer end of a glycogen chain to an alpha-1,6 position on the same or a neighboring glycogen chain. Branching of the chains is essential to increase the solubility of the glycogen molecule and, consequently, in reducing the osmotic pressure within cells. Highest level of this enzyme are found in liver and muscle. Mutations in this gene are associated with glycogen storage disease IV (also known as Andersen's disease). [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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ICC/IF Video Protocol

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Bioinformatics

Gene Symbol GBE1
Entrez