GATA-6 Antibody (2B3)

Images

 
Western Blot: GATA-6 Antibody (2B3) [NBP1-47766] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Gata6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunocytochemistry/ Immunofluorescence: GATA-6 Antibody (2B3) [NBP1-47766] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Gata6.
Immunohistochemistry-Paraffin: GATA-6 Antibody (2B3) [NBP1-47766] - Staining of paraffin-embedded Human lung tissue using anti-Gata6 mouse monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: GATA-6 Antibody (2B3) [NBP1-47766] - Staining of HT29 cells using anti-Gata6 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, MkSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
2B3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

GATA-6 Antibody (2B3) Summary

Immunogen
Recombinant protein expressed in E.coli corresponding to amino acids 300-449 of human GATA6
Specificity
This antibody is specific for Homo sapiens GATA binding protein 6 (GATA6).
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
GATA6
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Immunocytochemistry/Immunofluorescence 1:50-100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for GATA-6 Antibody (2B3)

  • GATA binding protein 6
  • GATA6
  • GATA-6
  • GATA-binding factor 6
  • GATA-binding protein 6
  • transcription factor GATA-6

Background

The GATA family of transcription factors, which contain zinc fingers in their DNA binding domain, have emerged as candidate regulators of gene expression in hematopoietic cells (Tsai et al., 1994 [PubMed 8078582]). GATA6 Thought to be important for regulating terminal differentiation and/or proliferation

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol GATA6
Entrez
Uniprot