Gastrin peptide Antibody

Images

 
Western Blot: Gastrin peptide Antibody [NB100-91780] - Analysis of Gastrin (M90) pAb at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:NIH-3T3 whole cell lysateLane3:H9C2 whole cell lysate
Immunohistochemistry-Paraffin: Gastrin peptide Antibody [NB100-91780] - Paraffin-embedded human colon carcinoma tissue.
Western Blot: Gastrin peptide Antibody [NB100-91780] - Extracts from NIH/3T3 cells.
Immunohistochemistry-Paraffin: Gastrin peptide Antibody [NB100-91780] - Analyzes of Gastrin (M90)pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB100-91780
    • Availability
      Product Discontinued

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Gastrin peptide Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 61-101 of Human Gastrin.
Specificity
Gastrin (M90) detects endogenous levels of Gastrin protein.
Clonality
Polyclonal
Host
Rabbit
Gene
GAST
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of Gastrin (M90) pAb in extracts from NIH/3T3 cells.
Immunohistochemistry (IHC) analyzes of Gastrin (M90) pAb in paraffin-embedded human colon carcinoma tissue.
Theoretical MW
12 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Gastrin peptide Lysate (NBL1-10978)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Gastrin peptide Antibody

  • GAS
  • gastrin

Background

Gastrin, which is normally formed by mucosal cells in the gastric antrum and by the D cells of the pancreatic islets, is a hormone whose main function is to stimulate secretion of HCl by the gastric mucosa. HCl, in turn, inhibits gastrin formation. Gastrin also stimulates smooth muscle contraction and increases blood circulation and water secretion in the stomach and intestine. Gastrin is regulated by epidermal growth factor in both mice and humans. Gastrin is excreted in excess by pancreatic tumors in the Zollinger- Ellison syndrome. Gastrin maps to human chromosome 17q-21. Gastrin- Releasing Peptide (GRP) stimulates the release of gastrin as well as other gastrointestinal hormones, in addition to acting as an autocrine growth factor for certain cell types. High levels of GRP are found in the human lung just after birth and levels decrease thereafter in parallel with the observed disease in a number of pulmonary neuroendocrine cells. GRP is known to promote lung tumorigenesis in model systems and, interestingly, is induced by retinoic acid. GRP is involved in several functions with the hypothalamus, and is thought to play a role in regulating pituitary hormone secretion. GRP maps to human chromosome 18q21.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol GAST
Entrez
Uniprot