Western Blot: GAPDH Antibody [NB100-2546] - (0.1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: GAPDH Antibody [NB100-2546] - analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 ...read more
Immunohistochemistry-Paraffin: GAPDH Antibody [NB100-2546] - Human Liver.
WB: Approx. 36 kDa band observed in human liver, testis and tonsil lysates and in mouse spleen lysates (calculated MW of 36.1 kDa band according to NP_002037.2). An approx. 37 kDa band observed in rat liver lysates (calculated MW of 35.8 kDa band according to NP_058704.1). IHC-P: liver shows textured cytoplasm staining in hepatocytes. IHC-Fr: Rat liver shows textured staining of cytoplasm in hepatocytes. Data obtained from a previous batch different goat).
36 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine.
Packaging, Storage & Formulations
Store at -20C. Avoid freeze-thaw cycles.
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
0.02% Sodium Azide
Immunogen affinity purified
Alternate Names for GAPDH Antibody
aging-associated gene 9 protein
GAPDPeptidyl-cysteine S-nitrosylase GAPDH
glyceraldehyde 3-phosphate dehydrogenase
Glyceraldehyde-3-phosphate dehydrogenase catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. A GAPD pseudogene has been mapped to Xp21-p11 and 15 GAPD-like loci have been identified.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for GAPDH Antibody (NB100-2546). (Showing 1 - 6 of 6 FAQs).
We need a loading control antibody for WB working with HeLa cell line, will this GAPDH antibody work for that?
Well GAPDH is expressed in HeLa cell lines but whether or not it is the best option for a loading control antibody depends on the size of the protein you are lookin at. GAPDH antibody is a good choice for low to mid MW targets as GAPDH detects around 35 kDa.
I want to know why GAPDH is used as a loading control antibody in WB techniques.
Our GAPDH antibody makes a good loading control because GAPDH is a housekeeping gene essential to metabolism that is globally expressed in most tissue types and cell lines.
I need a suitable loading control antibody that I can use in western blot of rat nerve extracts, which do you recommend?
This GAPDH antibody (NB300-221) is a good choice for a loading control antibody and has been cited in over 180 publications and used on rat lysates with good results.
I'm looking for a loading control between 24kDa - 65kDa for use in simple western, we tried an actin antibody we already had but it didn't work. What can you recommend?
Our GAPDH antibody NB300-221 has been validated in simple western and detects around 35 kDa so I think it would be a good choice for a simple western loading control antibody based on your size requirements.
What secondary antibody should I use for visualization of the GAPDH antibody?
This GAPDH antibody is raised in mouse so you would want to use an anti-mouse secondary with the dye of your choice.
Do you offer a smaller size for the GAPDH antibodies?
We offer sample sizes for many of our GAPDH loading control antibodies including NB300-221.
GAPDH: More than a housekeeping gene GAPDH is a 146kD tetramer glycolytic pathway metabolic enzyme composed of four 30-40 kDa subunits. It is responsible for reversibly phosphorylating its substrate glyceraldehyde 3-phosphate within the glycolytic pathway. Apart from its role in gl... Read full blog post.
The GAPDH Antibody in Western blot Assays The loading controls on our antibody database are widely used in gel electrophoresis and Western blotting studies. Products like the GAPDH antibody detect "housekeeping" proteins which are abundantly distributed in cells. This makes them useful for ch... Read full blog post.
New Primers Available for ChIP Procedures Novus has recently released six new primer sets designed for use in real-time PCR DNA amplification of housekeeping, silent, or heterochromatin associated proteins. Specifically, the primers are for GAPDH, RPL30, MyoD1, AFM, alpha Satellite, and Satel... Read full blog post.
The GAPDH Antibody: A ChIP Off the Old Block GAPDH antibodies are commonly used as a loading control, due to GAPDH's widespread function as a house-keeping protein. As a leading scientific research antibody supplier, Novus Biologicals offer a wide range of GAPDH antibody reagents and support pro... Read full blog post.
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