Gankyrin Antibody

Images

 
Western Blot: Gankyrin Antibody [H00005716-B01P] - Analysis of PSMD10 expression in K-562.
Immunocytochemistry/ Immunofluorescence: Gankyrin Antibody [H00005716-B01P] - Analysis of purified antibody to PSMD10 on HeLa cell. (antibody concentration 10 ug/ml)
Western Blot: Gankyrin Antibody [H00005716-B01P] - Analysis of PSMD10 expression in transfected 293T cell line by PSMD10 polyclonal antibody. Lane 1: PSMD10 transfected lysate(24.86 KDa). Lane 2: Non-transfected lysate.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Gankyrin Antibody Summary

Immunogen
PSMD10 (NP_002805.1, 1 a.a. - 226 a.a.) full-length human protein. MEGCVSNLMVCNLAYSGKLEELKESILADKSLATRTDQDSRTALHWACSAGHTEIVEFLLQLGVPVNDKDDAGWSPLHIAASAGRDEIVKALLGKGAQVNAVNQNGCTPLHYAASKNRHEIAVMLLEGGANPDAKDHYEATAMHRAAAKGNLKMIHILLYYKASTNIQDTEGNTPLHLACDEERVEEAKLLVSQGASIYIENKEEKTPLQVAKGGLGLILKRMVEG
Specificity
PSMD10 - proteasome (prosome, macropain) 26S subunit, non-ATPase, 10,
Isotype
IgG
Clonality
Polyclonal
Host
Mouse
Gene
PSMD10
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
Protein A purified

Notes

Quality control test: Antibody reactive against mammalian transfected lysate.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Gankyrin Antibody

  • 26S proteasome non-ATPase regulatory subunit 10
  • 26S proteasome regulatory subunit p28
  • ankyrin repeat protein
  • dJ889N15.2
  • gankyrin
  • hepatocellular carcinoma-associated protein p28-II
  • p28
  • p28(GANK)
  • proteasome (prosome, macropain) 26S subunit, non-ATPase, 10

Background

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. Two transcripts encoding different isoforms have been described. Pseudogenes have been identified on chromosomes 3 and 20. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol PSMD10
Entrez
Uniprot