Gankyrin Antibody (3F6)

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Western Blot: PSMD10 Antibody (3F6) [NBP2-02199] Analysis of extracts (35ug) from 9 different cell lines by using anti-PSMD10 monoclonal antibody.
Immunohistochemistry-Paraffin: PSMD10 Antibody (3F6) [NBP2-02199] - Staining of paraffin-embedded prostate tissue using anti-PSMD10 mouse monoclonal antibody.
Flow Cytometry: PSMD10 Antibody (3F6) [NBP2-02199] - Analysis of Jurkat cells, using anti-PSMD10 antibody, (Red) compared to a nonspecific negative control antibody (Blue).
Western Blot: PSMD10 Antibody (3F6) [NBP2-02199] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMD10 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunohistochemistry-Paraffin: PSMD10 Antibody (3F6) [NBP2-02199] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-PSMD10 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PSMD10 Antibody (3F6) [NBP2-02199] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-PSMD10 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PSMD10 Antibody (3F6) [NBP2-02199] - Staining of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-PSMD10 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PSMD10 Antibody (3F6) [NBP2-02199] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-PSMD10mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PSMD10 Antibody (3F6) [NBP2-02199] - Staining of paraffin-embedded Carcinoma of liver tissue using anti-PSMD10 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PSMD10 Antibody (3F6) [NBP2-02199] - Staining of paraffin-embedded Carcinoma of prostate tissue using anti-PSMD10mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PSMD10 Antibody (3F6) [NBP2-02199] - Staining of paraffin-embedded endometrium tissue using anti-PSMD10 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PSMD10 Antibody (3F6) [NBP2-02199] - Staining of paraffin-embedded Kidney tissue using anti-PSMD10mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PSMD10 Antibody (3F6) [NBP2-02199] - Staining of paraffin-embedded liver tissue using anti-PSMD10 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PSMD10 Antibody (3F6) [NBP2-02199] - Staining of paraffin-embedded Ovary tissue using anti-PSMD10 mouse monoclonal antibody.
Flow Cytometry: PSMD10 Antibody (3F6) [NBP2-02199] - Analysis of Hela cells, using anti-PSMD10 antibody, (Red) compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, IHC-P
Clone
3F6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Gankyrin Antibody (3F6) Summary

Immunogen
Full length human recombinant protein of human PSMD10 (NP_002805) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PSMD10
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Gankyrin Antibody (3F6)

  • 26S proteasome non-ATPase regulatory subunit 10
  • 26S proteasome regulatory subunit p28
  • ankyrin repeat protein
  • dJ889N15.2
  • gankyrin
  • hepatocellular carcinoma-associated protein p28-II
  • p28
  • p28(GANK)
  • proteasome (prosome, macropain) 26S subunit, non-ATPase, 10

Background

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits.Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. Two transcripts encoding different isoforms have been described. Pseudogenes have been identified on chromosomes 3 and 20.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PSMD10
Entrez