Recombinant Human GAMT Protein

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SDS-Page: GAMT Protein [NBP1-30287] - GAMT, 28.4 kDa (256aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
5 mg/ml

Order Details

Recombinant Human GAMT Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-236 of Human GAMT

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSAPSATPIF APGENCSPAW GAAPAAYDAA DTHLRILGKP VMERWETPYM HALAAAASSK GGRVLEVGFG MAIAASKVQE APIDEHWIIE CNDGVFQRLR DWAPRQTHKV IPLKGLWEDV APTLPDGHFD GILYDTYPLS EETWHTHQFN FIKNHAFRLL KPGGVLTYCN LTSWGELMKS KYSDITIMFE ETQVPALLEA GFRRENIRTE VMALVPPADC RYYAFPQMIT PLVTKG

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
GAMT
Purity
>95% pure by SDS-PAGE

Applications/Dilutions

Theoretical MW
28.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer (pH8.0) containing 1mM DTT,10% glycerol
Preservative
No Preservative
Concentration
5 mg/ml
Purity
>95% pure by SDS-PAGE

Alternate Names for Recombinant Human GAMT Protein

  • EC 2.1.1.2
  • guanidinoacetate N-methyltransferase
  • PIG2
  • TP53I2

Background

Guanidinoacetate N-methyltransferase isoform a, also known as GAMT is a methyltansferase that converts guanidoacetate to creatine, using S-adenosylmethionine as the methyl domor. This enzyme participates in the two-step synthesis of the compound creatine from the protein building blocks glycine, arginine, and methionine. It is involved in providing energy for muscle contraction, and is also important in nervous system functioning. Also GAMT is active in the liver, pancreas, and kidneys. Recombinant human GAMT, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol GAMT