gamma Tubulin Antibody (14C11)

Images

 
Western Blot: gamma Tubulin Antibody (14C11) [NB100-78488] - Cell extract from A431 cells (Lanes 1) or NIH3T3 cells (Lanes 2) was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal ...read more

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB100-78488
    • Availability
      Product Discontinued

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gamma Tubulin Antibody (14C11) Summary

Immunogen
Peptide-KLH
Marker
Centrosome Marker
Specificity
Tubulin-gamma (14C11)
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TUBG1
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
Application Notes
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 mug per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Positive Control
gamma Tubulin Lysate (NBL1-17444)

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.2)
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for gamma Tubulin Antibody (14C11)

  • gamma-1-tubulin
  • Gamma-tubulin complex component 1
  • GCP-1
  • TUBG
  • TUBGCP1
  • tubulin gamma-1 chain
  • tubulin, gamma 1
  • tubulin, gamma polypeptide

Background

gamma-tubulin is a 51 kD member of the tubulin superfamily and is conserved between yeast and mammals. This cytoskeletal protein is highly expressed in centrosomes and is thought to be involved in cytoskeletal organization and mitosis. gamma-tubulin is expressed at highest levels during prophase to metaphase and can be modified by phosphorylation. This protein has been shown to interact with alpha-tubulin, paxillin, and RAC GTPase activated protein 1. The 14C11 antibody has been shown to be useful for Western blotting of human, mouse and rat gamma-tubulin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for gamma Tubulin Antibody (NB100-78488)

Discover related pathways, diseases and genes to gamma Tubulin Antibody (NB100-78488). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Bioinformatics

Gene Symbol TUBG1
Entrez