gamma H2AX [p Ser139] Antibody (2F3) [FITC]

Images

 
Flow Cytometry: gamma H2AX [p Ser139] Antibody (2F3) [FITC] [NB100-78357] - Staurosporine-treated (37oC, 4 hours) (filled green histogram) or non-treated Molt-4 cells (opened purple histogram) stained with 2F3 FITC

Product Details

Summary
Product Discontinued
View other related gamma H2AX Primary Antibodies

Order Details


    • Catalog Number
      NB100-78357
    • Availability
      Product Discontinued

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gamma H2AX [p Ser139] Antibody (2F3) [FITC] Summary

Immunogen
Modified peptide (KATQASQEY)
Modification
p Ser139
Marker
DNA Double-strand break marker
Specificity
H2A.X (2F3) [Ser139]
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
H2AFX
Purity
Protein A or G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
Application Notes
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 mug antibody per 5 ml antibody dilution buffer for each mini-gel. For immunofluorescence microscopy: Use a dilution range of 1~4 microgram/ml. It is recommended that the reagent be titrated for optimal performance for each application.
Theoretical MW
15.145 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
gamma H2AX Lysate (NBL1-11424)

Reactivity Notes

Reacts with Ser139-phosphorylated H2A.X. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS (pH 7.2) and 0.2% (w/v) BSA
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Notes

Licensed to Novus Biologicals LLC under U.S. Patent Nos. 6,362,317 and 6,884,873.

Alternate Names for gamma H2AX [p Ser139] Antibody (2F3) [FITC]

  • H2A histone family, member X
  • H2A.X
  • H2a/x
  • H2AX histone
  • H2AXhistone H2A.x
  • YH2AX

Background

H2A.X is a 14 kD basal histone and a member of the H2 histone family. This nuclear protein is synthesized in the G1 and S phase of the cell cycle and is known to be important for recombination between immunoglobulin switch regions. H2A.X becomes phosphorylated on serine 139 after double-stranded DNA breaks. Phosphorylated H2A.X promotes DNA repair and maintains genomic stability. The 2F3 monoclonal antibody reacts with phosphorylated human H2A.X (Ser139) and has been shown to be useful for Western blotting and immunofluorescence.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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FAQs for gamma H2AX Antibody (NB100-78357). (Showing 1 - 1 of 1 FAQ).

  1. Is there any evidence for species cross-reactivity of the NB100-78357?
    • Although we do not have any testing data regarding the cross-reactivity of NB100-78357 with other proteins, I ran a BLAST search using the immunogen sequence and found that this only pulled back histone H2AX and no other proteins. As such the antibody is not likely to cross-react with other proteins, although we have not confirmed this in-house.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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gamma H2AX NB100-78357

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Blogs on gamma H2AX.

Further unraveling the role of gamma H2AX in DNA damage response
Our genome experiences a moderate amount of DNA damage in our cells on a daily basis.  This DNA damage can be in response to external environmental factors, or be a result of our internal metabolic processes going awry.  While normal rates of DNA ...  Read full blog post.

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Bioinformatics

Gene Symbol H2AFX