gamma H2AX [p Ser139] Antibody (2F3)

Images

 
Immunocytochemistry/ Immunofluorescence: gamma H2AX [p Ser139] Antibody (2F3) [NB100-78356] - ICC-IF staining of HeLa cells with purified gamma H2AX [p Ser139] antibody (clone 2F3) followed by labelling with DyLight 594 ...read more
Immunocytochemistry/ Immunofluorescence: gamma H2AX [p Ser139] Antibody (2F3) [NB100-78356] - Untreated Hela cells (Panel A), or overnight nocodazole treated Hela cells (Panel B) stained with purified mouse monoclonal ...read more
Western Blot: gamma H2AX [p Ser139] Antibody (2F3) [NB100-78356] - Untreated (Lane 1) and staurosporine-treated (Lane 2) Jurkat nuclear extract, untreated (Lane 3) and UV treated (Lane 4) Jurkat cell extract were ...read more
Flow Cytometry: gamma H2AX [p Ser139] Antibody (2F3) [NB100-78356] - Analysis using the FITC conjugate of NB100-78356. Staurosporine-treated (37oC, 4 hours) (filled green histogram) or non-treated Molt-4 cells (opened ...read more

Product Details

Summary
Product Discontinued
View other related gamma H2AX Primary Antibodies

Order Details


    • Catalog Number
      NB100-78356
    • Availability
      Product Discontinued

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gamma H2AX [p Ser139] Antibody (2F3) Summary

Immunogen
Modified peptide (KATQASQEY)
Modification
p Ser139
Marker
DNA Double-strand break marker
Specificity
H2A.X (2F3) [Ser139]
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
H2AFX
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1-4 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 mug antibody per 5 ml antibody dilution buffer for each mini-gel. For immunofluorescence microscopy: Use a dilution range of 1~4 microgram/ml. It is recommended that the reagent be titrated for optimal performance for each application. FC reported in literature (PMID:23449797).
Theoretical MW
15.145 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
gamma H2AX Lysate (NBL1-11424)
Reviewed Applications
Read 1 Review rated 4
using
NB100-78356 in the following applications:

Publications
Read Publications using
NB100-78356 in the following applications:

Reactivity Notes

Reacts with Ser139-phosphorylated H2A.X. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.2)
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Notes

Licensed to Novus Biologicals LLC under U.S. Patent Nos. 6,362,317 and 6,884,873.

Alternate Names for gamma H2AX [p Ser139] Antibody (2F3)

  • H2A histone family, member X
  • H2A.X
  • H2a/x
  • H2AX histone
  • H2AXhistone H2A.x
  • YH2AX

Background

H2A.X is a 14 kD basal histone and a member of the H2 histone family. This nuclear protein is synthesized in the G1 and S phase of the cell cycle and is known to be important for recombination between immunoglobulin switch regions. H2A.X becomes phosphorylated on serine 139 after double-stranded DNA breaks. Phosphorylated H2A.X promotes DNA repair and maintains genomic stability. The 2F3 monoclonal antibody reacts with phosphorylated human H2A.X (Ser139) and has been shown to be useful for Western blotting and immunofluorescence.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-78356
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P

Publications for gamma H2AX Antibody (NB100-78356)(23)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 5 applications: FLOW, ICC/IF, IHC, IHC-P, WB.


Filter By Application
FLOW
(2)
ICC/IF
(12)
IHC
(1)
IHC-P
(1)
WB
(4)
All Applications
Filter By Species
Human
(7)
All Species
Showing Publications 1 - 10 of 23. Show All 23 Publications.
Publications using NB100-78356 Applications Species
Jakl L, Lobachevsky P, Vokalova L et al. Validation of JCountPro software for efficient assessment of ionizing radiation-induced foci in Human lymphocytes Int. J. Radiat. Biol. Sep 20 2016 [PMID: 27648492] (ICC/IF, Human) ICC/IF Human
Wolf C, Rapp A, Berndt N et al. RPA and Rad51 constitute a cell intrinsic mechanism to protect the cytosol from self DNA. Nat Commun. May 27 2016 [PMID: 27230542] (ICC/IF, Human) ICC/IF Human
Onyango DO, Howard SM, Neherin K et al. Tetratricopeptide repeat factor XAB2 mediates the end resection step of homologous recombination. Nucleic Acids Res. 2016 Apr 15 [PMID: 27084940] (ICC/IF, WB, Human) ICC/IF, WB Human
Markova E, Somsedikova A, Vasilyev S et al. DNA repair foci and late apoptosis/necrosis in peripheral blood lymphocytes of breast cancer patients undergoing radiotherapy. Int. J. Radiat. Biol. 2015 Nov 04 [PMID: 26537244] (ICC/IF, Human) ICC/IF Human
Murakami N, Kuhnel A, Schmid TE et al. Role of membrane Hsp70 in radiation sensitivity of tumor cells. Radiat Oncol 2015 Jul 22 [PMID: 26197988] (FLOW, Human)

Details:
Using the Alexa Fluor (R) 488 conjugated version of NB100-78356, catalog number NB100-78356AF488.
FLOW Human
Gunther C, Kind B, Reijns MA et al. Defective removal of ribonucleotides from DNA promotes systemic autoimmunity. J. Clin. Invest. 2014 Dec 15 [PMID: 25500883] (IHC-P, Human) IHC-P Human
Camps J, Wangsa D, Falke M et al. Loss of lamin B1 results in prolongation of S phase and decondensation of chromosome territories. FASEB J. 2014 Apr 14 [PMID: 24732130] (ICC/IF, Human) ICC/IF Human
Assaily W, Rubinger DA, Wheaton K et al. ROS-Mediated p53 Induction of Lpin1 Regulates Fatty Acid Oxidation in Response to Nutritional Stress. Mol Cell 444(3):491-501. 2011 Nov. [PMID: 22055193]
Martinelli P et al. Blood. 117:6617. 2011 [PMID: 21518927]
Li M et al. Mol Cell Biol. 31:2090. 2011 [PMID: 21402780]
Show All 23 Publications.

Review for gamma H2AX Antibody (NB100-78356) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human and Mouse.
We have 1 review tested in 1 application: ICC.

Reviews using NB100-78356:
Filter by Applications
ICC
(1)
All Applications
Filter by Species
Human and Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry gamma H2AX NB100-78356
Enlarge
reviewed by:
Vincent Picco
ICC Human and Mouse 08/18/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedCultured cell lines
SpeciesHuman and Mouse
LotB219075

Comments

CommentsCell fixed in PBS-2% PFA and permeabilised in PBS-0.1% Triton X100.
Antibody used 1/300.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for gamma H2AX Antibody (NB100-78356) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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gamma H2AX NB100-78356

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Blogs on gamma H2AX.

Further unraveling the role of gamma H2AX in DNA damage response
Our genome experiences a moderate amount of DNA damage in our cells on a daily basis.  This DNA damage can be in response to external environmental factors, or be a result of our internal metabolic processes going awry.  While normal rates of DNA ...  Read full blog post.

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Recent Reviews

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4
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1
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Vincent Picco
08/18/2017
Application: ICC
Species: Human and Mouse

Bioinformatics

Gene Symbol H2AFX