Immunocytochemistry/ Immunofluorescence: gamma H2AX [p Ser139] Antibody (2F3) [NB100-78356] - ICC-IF staining of HeLa cells with purified gamma H2AX [p Ser139] antibody (clone 2F3) followed by labelling with DyLight 594 ...read more
Immunocytochemistry/ Immunofluorescence: gamma H2AX [p Ser139] Antibody (2F3) [NB100-78356] - Untreated Hela cells (Panel A), or overnight nocodazole treated Hela cells (Panel B) stained with purified mouse monoclonal ...read more
Western Blot: gamma H2AX [p Ser139] Antibody (2F3) [NB100-78356] - Untreated (Lane 1) and staurosporine-treated (Lane 2) Jurkat nuclear extract, untreated (Lane 3) and UV treated (Lane 4) Jurkat cell extract were ...read more
Flow Cytometry: gamma H2AX [p Ser139] Antibody (2F3) [NB100-78356] - Analysis using the FITC conjugate of NB100-78356. Staurosporine-treated (37oC, 4 hours) (filled green histogram) or non-treated Molt-4 cells (opened ...read more
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 mug antibody per 5 ml antibody dilution buffer for each mini-gel. For immunofluorescence microscopy: Use a dilution range of 1~4 microgram/ml. It is recommended that the reagent be titrated for optimal performance for each application. FC reported in literature (PMID:23449797).
15.145 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reacts with Ser139-phosphorylated H2A.X. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Packaging, Storage & Formulations
Store at 4C. Do not freeze.
PBS (pH 7.2)
0.09% Sodium Azide
Protein A or G purified
Licensed to Novus Biologicals LLC under U.S. Patent Nos. 6,362,317 and 6,884,873.
Alternate Names for gamma H2AX [p Ser139] Antibody (2F3)
H2A histone family, member X
H2A.X is a 14 kD basal histone and a member of the H2 histone family. This nuclear protein is synthesized in the G1 and S phase of the cell cycle and is known to be important for recombination between immunoglobulin switch regions. H2A.X becomes phosphorylated on serine 139 after double-stranded DNA breaks. Phosphorylated H2A.X promotes DNA repair and maintains genomic stability. The 2F3 monoclonal antibody reacts with phosphorylated human H2A.X (Ser139) and has been shown to be useful for Western blotting and immunofluorescence.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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