GADD153/CHOP Antibody

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Immunohistochemistry-Paraffin: GADD153/CHOP Antibody [NB100-92158] - Analysis of CHOP/GADD153 in paraffin-embedded human skelatal muscle tissue.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB100-92158
    • Availability
      Product Discontinued

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GADD153/CHOP Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids C-terminus of Human CHOP.
Marker
ER Stress Marker
Clonality
Polyclonal
Host
Rabbit
Gene
DDIT3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
Theoretical MW
19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
FUS Lysate (NBL1-10861)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GADD153/CHOP Antibody

  • C/EBP-homologous protein 10
  • C/EBP-homologous protein
  • CCAAT/enhancer-binding protein homologous protein
  • CEBP zeta
  • CHOP
  • CHOP10 CEBPZ
  • CHOP10
  • CHOP-10
  • CHOPC/EBP zeta
  • DDIT3
  • DDIT-3
  • DNA damage-inducible transcript 3 protein
  • DNA-damage-inducible transcript 3
  • GADD153
  • Growth arrest and DNA damage-inducible protein GADD153

Background

GADD153 is a small nuclear protein that is capable of dimerizing with transcription factors C/EBP alpha and beta. Once dimerized, this complex inhibits the normal binding and function of C/EBP to classical binding sites. Inversely, the C/EBP GADD153 dimer gains binding activity to other non classical C/EBP stress related targets. Under normal cellular conditions this protein is not expressed in detectable levels, but is highly unregulated during times of cellular/ER stress. Examples of GADD153 inducing stress include: treatment with tunicamycin, nutrient starvation and reducing agents that interfere with the calcium flux across the ER membrane.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GADD153/CHOP Antibody (NB100-92158). (Showing 1 - 2 of 2 FAQ).

  1. I am using NB100-92158. Can I use this antibody for western blot?
    • I am showing it has been successful in Western Blot testing. The band is detected approximately 19 kDa in Jurkat cells. The optimal dilution tested by our lab was 1:500-1:1000.
  2. What is the epitope for this antibody?
    • The immunogen sequence of NB100-92158 corresponds to amino acids 11-50 of the protein (UniProt P35638).

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Diseases for GADD153/CHOP Antibody (NB100-92158)

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PTMs for GADD153/CHOP Antibody (NB100-92158)

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Calculators

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Bioinformatics

Gene Symbol DDIT3
Entrez
Uniprot