GAD1/GAD67 Antibody

Images

 
Western Blot: GAD1/GAD67 Antibody [NB100-91776] - Analysis of GAD-67 (A492) pAb at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:NIH-3T3 whole cell lysateLane3:H9C2 whole cell lysate
Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody [NB100-91776] - Analyzes of GAD-67 (A492) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.
Western Blot: GAD1/GAD67 Antibody [NB100-91776] - Extracts from LOVO cells.
Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody [NB100-91776] - Paraffin-embedded human brain tissue.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB100-91776
    • Availability
      Product Discontinued

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GAD1/GAD67 Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 460-514 of Human GAD-67.
Clonality
Polyclonal
Host
Rabbit
Gene
GAD1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of GAD67 (A492) pAb in extracts from LOVO cells.
Immunohistochemistry (IHC) analyzes of GAD67 (A492) pAb in paraffin-embedded human brain tissue.
Theoretical MW
67 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
GAD1/GAD67 Lysate (NBL1-10933)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GAD1/GAD67 Antibody

  • 67kD)
  • EC 4.1.1
  • FLJ45882
  • GAD1
  • GAD25
  • GAD67
  • glutamate decarboxylase 1 (brain, 67kDa)
  • Glutamate decarboxylase 67 kDa isoform

Background

There are two forms of glutamic acid decarboxylases (GADs) that are found in the brain: GAD-65 (also known as GAD2) and GAD-67 (also known as GAD1, GAD or SCP). GAD-65 and GAD-67 are members of the group II decarboxylase family of proteins and are responsible for catalyzing the rate limiting step in the production of GABA (gamma-aminobutyric acid) from L-glutamic acid. Although both GADs are found in the brain, GAD-65 localizes to synaptic vesicle membranes in nerve terminals, while GAD-67 is distributed throughout the cell. GAD-67 is responsible for the basal levels of GABA synthesis. In the case of a heightened demand for GABA in neurotransmission, GAD-65 will transiently activate to assist in GABA production. The loss of GAD-65 is detrimental and can impair GABA neurotransmission, however the loss of GAD-67 is lethal. Due to alternative splicing, two isoforms exist for GAD-67: the predominant GAD-67 form and the minor GAD-25 form. GAD-25 is not expressed in brain but can be found in a variety of endocrine tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GAD1
Entrez