GAD1/GAD67 Antibody


Western Blot: GAD1/GAD67 Antibody [NB100-91776] - Analysis of GAD-67 (A492) pAb at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:NIH-3T3 whole cell lysateLane3:H9C2 whole cell lysate
Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody [NB100-91776] - Analyzes of GAD-67 (A492) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.
Western Blot: GAD1/GAD67 Antibody [NB100-91776] - Extracts from LOVO cells.
Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody [NB100-91776] - Paraffin-embedded human brain tissue.

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GAD1/GAD67 Antibody Summary

Synthetic peptide, corresponding to amino acids 460-514 of Human GAD-67.
Immunogen affinity purified
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  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of GAD67 (A492) pAb in extracts from LOVO cells.
Immunohistochemistry (IHC) analyzes of GAD67 (A492) pAb in paraffin-embedded human brain tissue.
Theoretical MW
67 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
GAD1/GAD67 Lysate (NBL1-10933)

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS (pH 7.2)
0.05% Sodium Azide
1.0 mg/ml
Immunogen affinity purified

Alternate Names for GAD1/GAD67 Antibody

  • 67kD)
  • EC 4.1.1
  • FLJ45882
  • GAD1
  • GAD25
  • GAD67
  • glutamate decarboxylase 1 (brain, 67kDa)
  • Glutamate decarboxylase 67 kDa isoform


There are two forms of glutamic acid decarboxylases (GADs) that are found in the brain: GAD-65 (also known as GAD2) and GAD-67 (also known as GAD1, GAD or SCP). GAD-65 and GAD-67 are members of the group II decarboxylase family of proteins and are responsible for catalyzing the rate limiting step in the production of GABA (gamma-aminobutyric acid) from L-glutamic acid. Although both GADs are found in the brain, GAD-65 localizes to synaptic vesicle membranes in nerve terminals, while GAD-67 is distributed throughout the cell. GAD-67 is responsible for the basal levels of GABA synthesis. In the case of a heightened demand for GABA in neurotransmission, GAD-65 will transiently activate to assist in GABA production. The loss of GAD-65 is detrimental and can impair GABA neurotransmission, however the loss of GAD-67 is lethal. Due to alternative splicing, two isoforms exist for GAD-67: the predominant GAD-67 form and the minor GAD-25 form. GAD-25 is not expressed in brain but can be found in a variety of endocrine tissues.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

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Gene Symbol GAD1