GABARAP Antibody

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Western Blot: GABARAP Antibody [NB110-74797] - rat brain lysate using Sheep antibody to human, rat, mouse GABARAP IgG at a concentration of 5 ug/ml under reducing condition.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB110-74797
    • Availability
      Product Discontinued

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GABARAP Antibody Summary

Immunogen
A synthetic peptide as a part of human, rat, mouse GABA(A) receptor-associated protein (GABARAP, MM46, FLC3B) conjugated to immunogenic carrier protein has been used as the immunogen.
Specificity
GABARAP.
Clonality
Polyclonal
Host
Sheep
Gene
GABARAP
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-10 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500

Reactivity Notes

Human, Mouse and Rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
LYOPH
Purity
IgG purified
Reconstitution Instructions
Reconstitute with 0.5 ml sterilized water. Add glycerol (1:1) for additional stability.

Alternate Names for GABARAP Antibody

  • Apg8p1
  • ATG8A
  • FLC3B
  • FLJ25768
  • GABA(A) receptor-associated proteinMGC120154
  • GABARAP
  • gamma-aminobutyric acid receptor-associated protein
  • MM46
  • MM46MGC120155

Background

Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. GABARAP belongs to a large family of proteins that mediate intracellular membrane trafficking and/or fusion. GABARAP binds not only to GABA, type A but also to tubulin, gephrin, and ULK1. May play a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. It interacts with GPHN and NSF; interacts with GABRG2, beta-tubulin and ULK1. SUBCELLULAR LOCATION: Intracytoplasmic membrane. Cytoplasm, cytoskeleton. Note: Largely associated with intracellular membrane structures including the Golgi apparatus and post-synaptic cisternae. Colocalizes with microtubules. TISSUE SPECIFICITY: Heart, brain, placenta, liver, skeletal muscle, kidney and pancreas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics Tool for GABARAP Antibody (NB110-74797)

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Diseases for GABARAP Antibody (NB110-74797)

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Research Areas for GABARAP Antibody (NB110-74797)

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Blogs on GABARAP.

Breakdown: Interpreting LC3 Antibody WB Results
In rodents, MAPLC3 (Microtubule-associated protein 1 light chain 3) is expressed in the renal visceral epithelial cells, or podocytes. LC3 antibody analysis has shown the protein accumulates in its membrane-bound form of LC3II, following conversion fr...  Read full blog post.

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Bioinformatics

Gene Symbol GABARAP
Entrez
Uniprot