GABA-AR alpha 5 Antibody

Western Blot: GABA-AR alpha 5 Antibody [NBP2-16567] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GABA A Receptor alpha 5 antibody [N2C2], Internal diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: GABA-AR alpha 5 Antibody [NBP2-16567] - Mouse cerebellum. GABA A Receptor alpha 5 stained by GABA A Receptor alpha 5 antibody [N2C2], Internal diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

GABA-AR alpha 5 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human GABA-AR alpha 5. The exact sequence is proprietary.
• Localization: Cell junction; synapse; postsynaptic cell membrane; Multi-pass membrane protein; Cell membrane; Multi-pass membrane protein
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GABRA5
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 52 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for GABA-AR alpha 5 Antibody

• GABA A R alpha 5
• GABA(A) receptor subunit alpha-5
• GABAAR alpha 5
• GABAARa5
• GABRA5
• gamma-aminobutyric acid (GABA) A receptor, alpha 5
• gamma-aminobutyric acid receptor subunit alpha-5
• MGC138184

Background

GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified. Transcript variants utilizing three different alternative non-coding first exons have been described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GABRA5
• Uniprot:
  • P31644(Human)