GABA-A R alpha 5 Antibody (S415-24)

Images

 
Western Blot: GABA-A R alpha 5 Antibody (S415-24) [NBP2-59700] - Analysis of Mouse Brain showing detection of ~55 kDa GABA A Receptor Alpha 5 protein using Mouse Anti-GABA A Receptor Alpha 5 Monoclonal Antibody, Clone ...read more
Immunocytochemistry/ Immunofluorescence: GABA-A R alpha 5 Antibody (S415-24) [NBP2-59700] - Analysis using Mouse Anti-GABA-A Receptor Alpha5 Monoclonal Antibody, Clone S415-24. Tissue: Neuroblastoma cell line SK-N-BE. ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF
Clone
S415-24
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

GABA-A R alpha 5 Antibody (S415-24) Summary

Immunogen
Fusion protein amino acids 368-419 (last cytoplasmic domain before extracellular C-terminus) of human GABA A Receptor Alpha5.
Localization
Cell Junction, Synapse, Postsynaptic Cell Membrane, Cell Membrane
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
GABRA5
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence
Application Notes
A 1:100 dilution of this antibody was sufficient for detection of GABA-A R, Alpha5 in 20 ug of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS pH7.4, 50% glycerol
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for GABA-A R alpha 5 Antibody (S415-24)

  • GABA A R alpha 5
  • GABA(A) receptor subunit alpha-5
  • GABAAR alpha 5
  • GABAARa5
  • GABRA5
  • gamma-aminobutyric acid (GABA) A receptor, alpha 5
  • gamma-aminobutyric acid receptor subunit alpha-5
  • MGC138184

Background

The GABA A Receptor is a member of the superfamily of fast acting ligand-gated ion channels. The individual subunits of these receptors have similar sequences and structural features (1). GABA-A receptors are the major fast inhibitory neurotransmitter gated ion channels in the brain (2). Recent research shows their usage in investigating side effects of drugs and the effects of learning and memory. Inverse agonists of the Alpha5 subunit may be useful in the treatment of Alzheimer's Disease (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol GABRA5
Entrez