GABA-A R alpha 1 Antibody

Images

 
Western Blot: GABA-A R alpha 1 Antibody [NB300-191] - Mouse forebrain lysates from Wild Type (WT) and alpha 1-knockout (K/O) animals showing specific immunolabeling of the -51 kDa alpha 1-subunit of the GABAA-R. The ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ca, PmSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.15 mg/ml

Order Details

GABA-A R alpha 1 Antibody Summary

Immunogen
Synthetic peptide corresponding to amino acid residues from the N-terminal region of the rat alpha1-subunit of GABA A Receptor conjugated to KLH
Localization
Membrane; multi pass membrane protein.
Specificity
Specific for the ~51k alpha1-subunit of the GABAA receptor in Western blots of rat brain. Labeling is absent in alpha1-subunit knockout animals.
Predicted Species
Bovine (100%), Canine (100%), Primate (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GABRA1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
Theoretical MW
51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NB300-191 in the following application:

Publications
Read Publications using
NB300-191 in the following applications:

  • 2 publications
  • IP
    2 publications
  • WB
    6 publications

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 22927941)

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Preservative
No Preservative
Concentration
0.15 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GABA-A R alpha 1 Antibody

  • ECA4
  • EJM
  • EJM5GABA(A) receptor subunit alpha-1
  • GABA A R alpha 1
  • GABAAR alpha 1
  • GABAARa1
  • GABRA1
  • gamma-aminobutyric acid (GABA) A receptor, alpha 1
  • gamma-aminobutyric acid receptor subunit alpha-1

Background

GABA (A) receptors are ligand-gated chloride channels that play a role as inhibitory neurotransmitters. They are known targets for certain classes of environmental and pharmaceutical compounds because a number of drugs interact with binding sites on GABA (A). Some of these drugs include benzodiazepines, anticonvulsants, and anesthetics. GABA (A) Receptors are comprised of subunits and these are further classified into 3 major groups (alpha, beta, and gamma). The subunits determine the pharmacological characteristics.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB300-190
Species: Hu, Mu, Rt
Applications: WB, IHC
NB300-198
Species: Mu, Rt
Applications: WB
NB300-199
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, IF, IHC-FrFl
NBP2-36560
Species: Mu, Rt
Applications: WB, IHC
NB300-196
Species: Mu, Rt
Applications: WB, ICC/IF
NB300-195
Species: Mu, Rt
Applications: WB, IHC
AF2086
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC
640-A3
Species: Mu
NBP1-87499
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NB110-41404
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC
NBP1-85585
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-58876
Species: Hu
Applications: ICC/IF
AF2180
Species: Hu
Applications: WB, Simple Western, IP
NBP2-38245
Species: Hu
Applications: IHC, IHC-P
NBP1-33284
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-04663
Species: Hu
NBP1-81838
Species: Hu, Mu
Applications: WB, IHC, IHC-P
H00006326-Q01
Species: Hu
248-BD/CF
Species: Hu, Mu, Rt, Ca, Eq
NB300-191
Species: Hu, Mu, Rt, Bv, Ca, Pm
Applications: WB

Publications for GABA-A R alpha 1 Antibody (NB300-191)(10)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: ICC/IF, IP, WB.


Filter By Application
ICC/IF
(2)
IP
(2)
WB
(6)
All Applications
Filter By Species
Human
(1)
Mouse
(4)
Rat
(7)
All Species
Showing Publications 1 - 10 of 10.
Publications using NB300-191 Applications Species
Popoola DO, Nizhnikov ME, Cameron NM. Strain-specific programming of prenatal ethanol exposure across generations. Alcohol: An International Biomedical Journal 2017 Jan 03 [PMID: 28433421] (WB, Rat) WB Rat
Carlson SL, O'Buckley TK, Thomas R et al. Altered GABAA Receptor Expression and Seizure Threshold Following Acute Ethanol Challenge in Mice Lacking the RIIbeta Subunit of PKA. Neurochem Res. 2013 Oct 9 [PMID: 24104609] (WB, Mouse) WB Mouse
Mendu SK, Bhandage A, Jin Z, Birnir B. Different Subtypes of GABA-A Receptors Are Expressed in Human, Mouse and Rat T Lymphocytes PLoS One 2012 [PMID: 22927941] (ICC/IF, Rat, Mouse, Human) ICC/IF Rat, Mouse, Human
Lin TY, Lu CW, Wang CC et al. Hispidulin inhibits the release of glutamate in rat cerebrocortical nerve terminals Toxicol Appl Pharmacol 2012 Jul 1 [PMID: 22759588] (WB, Rat) WB Rat
Kumar S, Ren Q, Beckley JH, O'Buckley TK, Gigante ED, Santerre JL, Werner DF, Morrow AL. Ethanol Activation of Protein Kinase A Regulates GABA(A) Receptor Subunit Expression in the Cerebral Cortex and Contributes to Ethanol-Induced Hypnosis. Front Neurosci;6:44. 2012 [PMID: 22509146] (WB, Rat) WB Rat
Suryanarayanan A, Liang J, Meyer EM et al. Subunit Compensation and Plasticity of Synaptic GABA(A) Receptors Induced by Ethanol in ?4 Subunit Knockout Mice. Front Neurosci;5:110. 2011 [PMID: 21977012] (WB, Mouse) WB Mouse
Shen Y, Lindemeyer AK, Spigelman I et al. Plasticity of GABAA receptors after ethanol pre-exposure in cultured hippocampal neurons Mol Pharmacol 2011 Mar [PMID: 21163967] (WB, Mouse) WB Mouse
Kumar S, Suryanarayanan A, Boyd KN et al. Ethanol reduces GABAA alpha1 subunit receptor surface expression by a protein kinase Cgamma-dependent mechanism in cultured cerebral cortical neurons. Mol Pharmacol;77(5):793-803. 2010 May. [PMID: 20159950] (ICC/IF, Rat) ICC/IF Rat
Marutha Ravindran CR, Mehta AK, Ticku MK. Effect of chronic administration of ethanol on the regulation of tyrosine kinase phosphorylation of the GABAA receptor subunits in the rat brain. Neurochem Res 2007 Jul [PMID: 17404840] (IP, Rat) IP Rat
Mehta AK, Ticku MK. Prevalence of the GABAA receptor assemblies containing alpha1-subunit in the rat cerebellum and cerebral cortex as determined by immunoprecipitation: lack of modulation by chronic ethanol administration. Brain Res Mol Brain Res;67(1):194-9. 1999 Apr 6. [PMID: 10101248] (IP, Rat) IP Rat

Review for GABA-A R alpha 1 Antibody (NB300-191) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Rat.
We have 1 review tested in 1 application: WB.

Reviews using NB300-191:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot GABA-A R alpha 1 NB300-191
Enlarge
reviewed by:
Daniel Popoola
WB Rat 03/21/2017
View

Summary

ApplicationWestern Blot
Sample Tested brain and spinal cord,brain
SpeciesRat

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for GABA-A R alpha 1 Antibody (NB300-191). (Showing 1 - 1 of 1 FAQs).

  1. Are there neutralizing peptides for this antibody?
    • There is no neutralizing peptide available for this antibody.

Secondary Antibodies

 

Isotype Controls

Additional GABA-A R alpha 1 Products

Bioinformatics Tool for GABA-A R alpha 1 Antibody (NB300-191)

Discover related pathways, diseases and genes to GABA-A R alpha 1 Antibody (NB300-191). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for GABA-A R alpha 1 Antibody (NB300-191)

Discover more about diseases related to GABA-A R alpha 1 Antibody (NB300-191).
 

Pathways for GABA-A R alpha 1 Antibody (NB300-191)

View related products by pathway.

PTMs for GABA-A R alpha 1 Antibody (NB300-191)

Learn more about PTMs related to GABA-A R alpha 1 Antibody (NB300-191).
 

Research Areas for GABA-A R alpha 1 Antibody (NB300-191)

Find related products by research area.

Blogs on GABA-A R alpha 1

There are no specific blogs for GABA-A R alpha 1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Daniel Popoola
03/21/2017
Application: WB
Species: Rat

Bioinformatics

Gene Symbol GABRA1
Entrez
Uniprot