Recombinant G-CSF Protein



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Recombinant G-CSF Protein Summary

A partial protein corresponding to CSF3.
E. coli-expressed, non-glycosylated protein consisting of amino acids Thr31-Pro204 of mature human G-CSF (accession # NM_172219).
Details of Functionality
The ED50 measured in a NFS-60 proliferation assay is typically 45 pg/ml, corresponding to a specific activity of approximately 2 x10^7Units/mg.
Protein/Peptide Type
Partial Recombinant Protein
Endotoxin Note
Less than 0.01 ng/ug cytokine as determined by the LAL assay.


Application Notes
This reagent has been tested in bioassays using the mouse cell line NFS-60.

Reactivity Notes

This protein was made to the human sequence.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
Sterile liquid; 10mM Na acetate, 0.4M NaCl, pH 4.0, 0.22 um filtered.
0.09% Sodium Azide


Purity: Greater than 99%, as determined by SDS-PAGE

Alternate Names for Recombinant G-CSF Protein

  • C17orf33
  • chromosome 17 open reading frame 33
  • colony stimulating factor 3 (granulocyte)
  • CSF3
  • CSF3OS
  • Filgrastim
  • GCSF
  • G-CSF
  • GCSFlenograstim
  • granulocyte colony-stimulating factor
  • Lenograstim
  • MGC45931
  • pluripoietin


Granulocyte Colony Stimulating Factor (G-CSF) is a member of the IL-6 cytokine family. It is 207 amino acids long with one glycosylation site and two disulfide bonds. G-CSF can be expressed by many different cell types including fibroblasts, endothelial cells, monocytes, macrophages and bone marrow stromal cells. Production of G-CSF is upregulated in response to infection and inflammatory mediators such as IL-1beta, TNFalpha, and LPS. IL-17 has also been shown to regulate the production of G-CSF. The receptor for G-CSF is structurally similar to type I cytokine receptors. The receptor is expressed on both hematopoietic and non-hematopoietic cells. Within the hematopoietic cells, G-CSF receptor is expressed predominantly on cells of myeloid origin, including granulocytes and monocytes but not on erythrocytes, lymphocytes or eosinophils. G-CSF receptor activates the Jak/STAT signaling pathway. G-CSF activates and mobilizes granulocytic precursors from the bone marrow and supports the proliferation, activation and differentiation of neutrophils in the blood.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Gene Symbol CSF3