Fumarase Antibody (OTI9G4)


Western Blot: Fumarase Antibody (9G4) [NBP1-47753] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-Fumarase monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; ...read more
Immunocytochemistry/ Immunofluorescence: Fumarase Antibody (9G4) [NBP1-47753] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Fumarase.
Immunohistochemistry-Paraffin: Fumarase Antibody (9G4) [NBP1-47753] - Staining of paraffin-embedded Human endometrium tissue using anti-Fumarase mouse monoclonal antibody.
Flow Cytometry: Fumarase Antibody (9G4) [NBP1-47753] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Fumarase antibody, and then ...read more
Western Blot: Fumarase Antibody (9G4) [NBP1-47753] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Fumarase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell ...read more
Immunohistochemistry-Paraffin: Fumarase Antibody (9G4) [NBP1-47753] - Staining of paraffin-embedded Human breast tissue using anti-Fumarase mouse monoclonal antibody.

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Fumarase Antibody (OTI9G4) Summary

Protein expressed in 293T cell transfected with human FH expression vector
This antibody is specific for Homo sapiens fumarate hydratase (FH), nuclear gene encoding mitochondrial protein.
Immunogen affinity purified
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  • Western Blot 1:1000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50
Positive Control
Fumarase Lysate (NBP2-65901)

Reactivity Notes

Human, Canine, Rat

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
0.02% Sodium Azide
Immunogen affinity purified

Alternate Names for Fumarase Antibody (OTI9G4)

  • EC
  • fumarase
  • fumarate hydratase
  • fumarate hydratase, mitochondrial
  • LRCC
  • MCL
  • MCUL1


The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form,differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion,where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

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Gene Symbol FH