Fumarase Antibody (1F10)

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Western Blot: Fumarase Antibody (1F10) [NBP1-47754] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-Fumarase monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; ...read more
Immunocytochemistry/ Immunofluorescence: Fumarase Antibody (1F10) [NBP1-47754] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Fumarase.
Immunohistochemistry-Paraffin: Fumarase Antibody (1F10) [NBP1-47754] - Staining of paraffin-embedded Human Kidney tissue using anti-Fumarase mouse monoclonal antibody.
Flow Cytometry: Fumarase Antibody (1F10) [NBP1-47754] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Fumarase antibody, and then ...read more
Western Blot: Fumarase Antibody (1F10) [NBP1-47754] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Fumarase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell ...read more
Immunocytochemistry/ Immunofluorescence: Fumarase Antibody (1F10) [NBP1-47754] - Staining of paraffin-embedded Human colon tissue using anti-Fumarase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Fumarase Antibody (1F10) [NBP1-47754] - Staining of paraffin-embedded Human endometrium tissue using anti-Fumarase mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Rt, CaSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
1F10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Fumarase Antibody (1F10) Summary

Immunogen
Full length human recombinant protein of human FH (NP_000134) produced in HEK293T cell.
Specificity
This antibody is specific for Homo sapiens fumarate hydratase (FH), nuclear gene encoding mitochondrial protein.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FH
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Fumarase Antibody (1F10)

  • EC 4.2.1.2
  • fumarase
  • fumarate hydratase
  • fumarate hydratase, mitochondrial
  • HLRCC
  • LRCC
  • MCL
  • MCUL1

Background

The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form,differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion,where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Fumarase Antibody (NBP1-47754). (Showing 1 - 1 of 1 FAQ).

  1. I'm going to be using this antibody for Immunohistochemistry. Can I use a secondary antibody directly conjugated with the HRP, without the biotin/avidin system? Would the sensitivity be drastically affected? The PBS working concentration is 1X, right (PBS 1X + 0.1% Triton X-100, pH 7.4)? Which will be the same used for primary antibody? Which is the most adequate HIER protocol for this antibody? Can you provide a list of references where they show satisfactory results using this antibody?
    • You may use NBP1-47754 with a secondary that is directly conjugated to HRP, but the sensitivity will be reduced. Using a biotin/strepavidin system allows greater staining essentially because multiple enzymes associate with each primary antibody. If you already have an HRP-conjugated secondary, I would start with that but keep in mind that you may need to change your detection system if you get no signal or weak staining. I'm sorry but we don't have any peer-reviewed references for this product. Here is the recommended protocol for antigen retrieval: Antigen Retrieval Solution: 0.01M Sodium Citrate Buffer, pH 6.0 To prepare stock solutions: Solution A. 0.1 M citric acid solution: dissolve 21.0 g of citric acid, monohydrate (C6H8O7.H2O) in 100 ml of dH2O. Solution B. 0.1M sodium citrate solution: dissolve 29.4 g trisodium citrate dihydrate (C6H5Na3O7.2H2O) in 100 ml of dH2O. Working solution: Add 9 ml of Stock solution A and 41 ml of stock solution B to 450 ml of dH2O. Adjust pH to 6.0.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol FH
Entrez
Uniprot