FTbeta Antibody

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Western Blot: FTbeta Antibody [NBP1-00836] Extracts from pc12 and raw264.7 cells.
Western Blot: FTbeta Antibody [NBP1-00836] - Western blot (WB) analysis of FTâ (E285) antibody in extracts from RAW264.7 cells.

Product Details

Summary
Product Discontinued
View other related FTbeta Primary Antibodies

Order Details


    • Catalog Number
      NBP1-00836
    • Availability
      Product Discontinued

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FTbeta Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 250-300 of Human FTbeta.
Specificity
FTbeta (E285) detects endogenous levels of FTbeta protein.
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
Application Notes
Western blot (WB) analysis of FTBeta (E285) pAb in extracts from pc12 and raw264.7 cells.
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Cross reacts with Human, Mouse and Rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Background

Mammalian protein farnesyl transferases are heterodimeric proteins containing two nonidentical alpha and beta subunits that attach farnesyl residues to a cysteine at the fourth position from the COOH terminus of several proteins, including nuclear lamins and p21Ras proteins. The natural substrates contain the Cys-A-A-Xaa recognition sequence, where the A residues are aliphatic and Xaa represents methionine, serine, glutamine or cysteine. The purified farnesyl transferase is an alpha-beta heterodimer. The beta subunit binds the peptide substrate while the alpha subunit is suspected to participate in formation of a stable complex with the substrate farnesyl pyrophosphate. The alpha subunit is shared with a second prenyl transferase, geranyl-geranyl transferase, that attaches 20 carbon geranylgeranyl to Ras related proteins that terminate in a Cys-A-A-Xaa recognition site in which Xaa is leucine.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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