FSH beta Antibody (OTI2A6)

Western Blot: FSH beta Antibody (2A6) [NBP2-02909] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FSH beta (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FSH beta.

Immunocytochemistry/Immunofluorescence: FSH beta Antibody (2A6) [NBP2-02909] Staining of COS7 cells transiently transfected by pCMV6-ENTRY FSH beta.

Flow Cytometry: FSH beta Antibody (2A6) [NBP2-02909] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-FSH beta antibody, and then analyzed by flow cytometry.

• Reactivity: Hu, Mu
• Applications: WB, Flow, ICC/IF
• Clone: OTI2A6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.42 mg/ml

FSH beta Antibody (OTI2A6) Summary

• Immunogen: Full length human recombinant protein of human FSHB (NP_001018090) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FSHB
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:500
• Theoretical MW: 12.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.42 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for FSH beta Antibody (OTI2A6)

• follicle stimulating hormone, beta polypeptide
• Follicle-stimulating hormone beta subunit
• Follitropin beta chain
• follitropin subunit beta
• Follitropin
• follitropin, beta chain
• FSH beta
• FSHB
• FSH-B
• FSH-beta

Background

The pituitary glycoprotein hormone family includes follicle-stimulating hormone, luteinizing hormone, chorionic gonadotropin, and thyroid-stimulating hormone. All of these glycoproteins consist of an identical alpha subunit and a hormone-specific beta subunit. This gene encodes the beta subunit of follicle-stimulating hormone. In conjunction with luteinizing hormone, follicle-stimulating hormone induces egg and sperm production. Alternative splicing results in two transcript variants encoding the same protein. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FSHB
• Entrez:
  • 2488(Human)