FOXO4 [p Ser197] Antibody


Western Blot: FOXO4 Antibody [NBP1-51418] - Extracts from COLO205 cells.
Immunohistochemistry-Paraffin: FOXO4 Antibody [NBP1-51418] - Human brain tissue.

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FOXO4 [p Ser197] Antibody Summary

Synthetic phosphopeptide derived from human FOXO4 around the phosphorylation site of Serine 197.
p Ser197
p-AFX1 (S197) pAb detects endogenous levels of AFX1 protein only when phosphorylated at S197.
Immunogen affinity purified
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  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analyzes of p-AFX1 (S197) pAb in extracts from COLO205 cells.
Immunohistochemistry (IHC) analyzes of p-AFX1 (S197) pAb in paraffin-embedded human brain tissue.
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, Mouse

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS (pH 7.2)
0.05% Sodium Azide
1.0 mg/ml
Immunogen affinity purified

Alternate Names for FOXO4 [p Ser197] Antibody

  • AFX
  • AFX1myeloid/lymphoid or mixed-lineage leukemia (trithorax (Drosophila) homolog);translocated to, 7
  • Fork head domain transcription factor AFX1
  • forkhead box O4
  • forkhead box protein O4
  • MLLT7MGC120490
  • myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila);translocated to, 7


FKHR (for forkhead in rhabdomyosarcoma), FKHRL1, and AFX1 are members of a subfamily of the forkhead family of transcription factors. AFX1, also known as FoxO4, is expressed in a wide variety of tissues and, like other FKHR proteins, AFX1 contains a single forkhead domain and serine-proline-rich region, which mediate DNA binding. AFX1-mediated transcriptional activation is regulated by the serine/threonine kinase Akt1, which phosphorylates AFX1 (in humans, this phosphorylation can occur at several sites, including Ser 197) and in turn, sequesters AFX1 in the cytosol, thereby blocking nuclear localization and DNA binding. Genetic mutations in FKHR genes, including the t(2;13) and t(1;3) translocations, are commonly found in alveolar rhabdomyosarcomas. Additionally, the t(x;11) translocation of the AFX1 gene, which involves the fusion of a serine-proline-rich sequence of AFX1 to the carboxyterminus of a truncated MLL, results in acute lymphocytic leukemia.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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The Wise Old Fox: Forkhead Transcription Factors and Age-Related DAF-16 Studies
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Gene Symbol FOXO4