FOXO3 Antibody

Western Blot: FOXO3 Antibody [NB100-1438] - (0.3ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer) with (A) and without (B) blocking with the immunising peptide. Detected by chemiluminescence.

Immunohistochemistry: FOXO3 Antibody [NB100-1438] - (5ug/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Control: FOXO3 Antibody [NB100-1438] - Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.

Product Discontinued

FOXO3 Antibody Summary

• Immunogen: Peptide with sequence C-GAKQASSQSWVPG corresponding to C-Terminus according to NP_001446.1.
• Localization: Nuclear
• Specificity: Both variants represent identical protein (NP_001446.1and NP_963853.1).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: FOXO3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Control
  • Immunohistochemistry 5 ug/ml
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 0.1 - 1 ug/ml
• Application Notes: WB: Approx. 90 kDa band observed in human brain and heart lysates (calculated MW of 71.3 kDa band according to NP_001446 and NP_963853).
• Control:

  FOXO3 Overexpression Lysate
  Human Heart Whole Tissue Lysate (Adult Whole Normal)
  Human Brain Whole Tissue Lysate (Adult Whole Normal)

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Porcine, Bovine.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris (pH 7.3), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for FOXO3 Antibody

• AF6q21 protein
• AF6q21
• DKFZp781A0677
• FKHRL1
• FKHRL1MGC31925
• FKHRL1P2
• forkhead box O3
• forkhead box protein O3
• forkhead homolog (rhabdomyosarcoma) like 1
• Forkhead in rhabdomyosarcoma-like 1
• forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1
• FOXO2
• FoxO3
• FOXO3A
• FOXO3Aforkhead box O3A
• MGC12739

Background

FOXO3A belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FOXO3 Antibody (NB100-1438)(1)

Publication using NB100-1438

• Brunet A, Bonni A, Zigmond MJ et al. Akt promotes cell survival by phosphorylating and inhibiting a Forkhead transcription factor. Fusion with MLL in leukemia Cell 1999-03-19 [PMID: 10102273]

Bioinformatics

• Gene Symbol: FOXO3
• Entrez:
  • 2309(Human)
  • 56484(Mouse)
• Uniprot:
  • O43524(Human)