FoxO1/FKHR Antibody

Images

 
Western Blot: FoxO1/FKHR Antibody [NBP1-77172] - Hela cell lysate with FOXO1A antibody at 1 ug/mL.
Immunocytochemistry/ Immunofluorescence: FoxO1/FKHR Antibody [NBP1-77172] - HeLa cells with FOXO1A antibody at 4 ug/mL.

Product Details

Summary
Product Discontinued
View other related FoxO1/FKHR Primary Antibodies

Order Details


    • Catalog Number
      NBP1-77172
    • Availability
      Product Discontinued

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FoxO1/FKHR Antibody Summary

Immunogen
Antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human FOXO1. The immunogen is located within the first 50 amino acids of FOXO1.
Clonality
Polyclonal
Host
Rabbit
Gene
FOXO1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000

Reactivity Notes

Human, Mouse, Rat

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FoxO1/FKHR Antibody

  • FKH1
  • FKHR
  • FKHRforkhead box protein O1
  • forkhead box O1
  • Forkhead box protein O1A
  • forkhead homolog in rhabdomyosarcoma
  • Forkhead in rhabdomyosarcoma
  • FoxO1
  • FOXO1Aforkhead, Drosophila, homolog of, in rhabdomyosarcoma

Background

FOXO1, also known as FKHR or ForkHead in Rhabdomyosarcoma, is a 70 kDa protein which is a ubiquitously expressed member of a subfamily of the forkhead homeotic gene family of transcription factors and shuttles between the cytoplasm and nucleus. FOXO transcription factors are key players of cell fate decisions, metabolism, stress resistance, tumor suppression and are regulated by growth factors, oxidative stress or nutrient deprivation. In insulin-responsive tissues, stress or nutrient abundance triggers phosphorylation by PKB/AKT, blocking nuclear translocation and activity. Genetic mutations involving FOXO1A are a cause of alveolar rhabdomyosarcoma (RMS2). Recent studies link the anti-tumor activity of FOXO1 and the process of autophagy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional FoxO1/FKHR Products

Bioinformatics Tool for FoxO1/FKHR Antibody (NBP1-77172)

Discover related pathways, diseases and genes to FoxO1/FKHR Antibody (NBP1-77172). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for FoxO1/FKHR Antibody (NBP1-77172)

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Pathways for FoxO1/FKHR Antibody (NBP1-77172)

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PTMs for FoxO1/FKHR Antibody (NBP1-77172)

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Research Areas for FoxO1/FKHR Antibody (NBP1-77172)

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Blogs on FoxO1/FKHR.

FOXO1/FKHR (fork head in Rhabdomyosarcoma)
FOXO1 belongs to the very large Forkhead family of transcription factors which contain a conserved distinct DNA-binding domain known as the Forkhead Box, or FOX. The Forkhead domain is a 100 amino acid long motif capable of binding and bending DNA,...  Read full blog post.

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Bioinformatics

Gene Symbol FOXO1
Entrez
Uniprot